SAFU Department, Regina Elena Cancer Institute, Rome, Italy.
J Vet Intern Med. 2011 Mar-Apr;25(2):407-11. doi: 10.1111/j.1939-1676.2011.0678.x. Epub 2011 Feb 11.
Electrochemotherapy (ECT) couples the administration of anticancer drugs with the delivery of electric pulses that increase the drug uptake through the cell membranes, resulting in an improved efficacy.
To evaluate the tolerability and efficacy of cisplatin (CDDP) as an ECT agent to prevent recurrence of incompletely resected mast cell tumors (MCTs).
Thirty-seven dogs.
Prospective study recruiting dogs with incompletely excised MCTs as confirmed by surgeon and pathology reports. After debulking, the tumor bed and margins were infiltrated with CDDP, and then exposed to trains of biphasic electrical pulses under sedation. Five minutes after the injection of the chemotherapy agent, sequences of 8 biphasic pulses lasting 50 + 50 μs each, were delivered in bursts of 1,300 V/cm for sclerosed and of 800 V/cm for exposed lesions, with caliper or needle array electrodes, respectively. A second session was performed 1 or 2 weeks later based on clinical considerations.
The treatment was well tolerated with minimal adverse effects. Twenty-nine dogs had no evidence of recurrence over the 6-year study period, 6 had tumor recurrence, 1 died of multiple cutaneous MCTs, and 1 died of unrelated causes. The estimated median time to recurrence was 1,200 days. Recurrence was not observed among the long-term (> 1 year) treated dogs.
ECT with CDDP appears effective in the treatment of incompletely resected MCT in dogs and could be a useful addition to the current options based on its low cost, limited toxicity, and ease of administration.
电化疗（ECT）将抗癌药物与电脉冲联合使用，电脉冲通过细胞膜增加药物摄取，从而提高疗效。
评估顺铂（CDDP）作为 ECT 药物预防不完全切除的肥大细胞瘤（MCT）复发的耐受性和疗效。
37 只狗。
这项前瞻性研究招募了经外科医生和病理报告证实不完全切除的 MCT 的狗。在肿瘤减积后，用 CDDP 浸润肿瘤床和边缘，然后在镇静下用双相电脉冲进行电穿孔。化疗药物注射后 5 分钟，用卡尺或针状电极分别以 1300 V/cm 为硬化病变和 800 V/cm 为暴露病变的强度传递持续 50 + 50 μs 的 8 个双相脉冲序列，每个脉冲序列持续 50 + 50 μs。根据临床情况，可在 1 或 2 周后进行第二次治疗。
治疗耐受性良好，副作用极小。29 只狗在 6 年的研究期间无复发迹象，6 只狗出现肿瘤复发，1 只死于多发性皮肤 MCT，1 只死于无关原因。复发的估计中位时间为 1200 天。在长期（> 1 年）治疗的狗中没有观察到复发。
CDDP 的 ECT 似乎对治疗狗的不完全切除的 MCT 有效，并且由于其成本低、毒性有限且易于管理，可能是当前治疗方案的有用补充。
