Spugnini Enrico P, Vincenzi Bruno, Amadio Bruno, Baldi Alfonso
Biopulse srl, Naples, Italy.
Medical Oncology Department, University Campus Bio-Medico of Rome, Rome, Italy.
Open Vet J. 2019 Apr;9(1):88-93. doi: 10.4314/ovj.v9i1.15. Epub 2019 Mar 25.
Electrochemotherapy (ECT) couples the administration of anticancer drugs with the delivery of electric pulses that increase the drug uptake through the cell membranes, thus resulting in an improved efficacy. This study has evaluated the tolerability and efficacy of the combination of systemic bleomycin and local cisplatin as ECT agents for incompletely excised canine soft tissue sarcoma (STS). Thirty dogs with incompletely excised STSs were enrolled. The dogs received intravenous 20 mg/m bleomycin, and the tumor bed and margins were infiltrated with cisplatin at the dose of 0.5 mg/cm. Then, trains of permeabilizing biphasic electric pulses were applied under sedation. More precisely, 5 min after the injection of the chemotherapy agents, sequences of eight biphasic pulses lasting 50 + 50 μsec each, were delivered in bursts of 1,300 V/cm using caliper electrodes. A second session was performed 2 wk later. The treatment was well tolerated and side effects were minimal. Twenty-six dogs had no evidence of recurrence at the time of manuscript writing; four had recurrence and one of the four recurring dogs died of lung metastases. Median estimated disease free was 857 d. Perivascular wall tumors response was compared to that of the other STSs, but the difference in outcome was not significant. ECT using combination of bleomycin and cisplatin appears to be effective in the treatment of incompletely resected STSs in dogs. This therapeutic approach could be a useful addition to the current options in consideration of its low cost, limited toxicity, and ease of administration.
电化学疗法（ECT）将抗癌药物的给药与电脉冲传递相结合，电脉冲可增加药物通过细胞膜的摄取，从而提高疗效。本研究评估了全身使用博来霉素和局部使用顺铂联合作为ECT药物治疗犬软组织肉瘤（STS）切除不完全病例的耐受性和疗效。纳入了30只STS切除不完全的犬。这些犬静脉注射20mg/m²博来霉素，肿瘤床和边缘以0.5mg/cm²的剂量注射顺铂。然后，在镇静状态下施加透化双相电脉冲序列。更确切地说，在注射化疗药物5分钟后，使用卡尺电极以1300V/cm的强度，每次持续50 + 50微秒的8个双相脉冲序列成组发放。2周后进行第二次治疗。该治疗耐受性良好，副作用最小。在撰写本文时，26只犬没有复发迹象；4只犬复发，其中1只复发犬死于肺转移。估计无病生存期的中位数为857天。将血管周围壁肿瘤的反应与其他STS的反应进行比较，但结果差异不显著。使用博来霉素和顺铂联合的ECT似乎对治疗犬切除不完全的STS有效。考虑到其成本低、毒性有限且易于给药，这种治疗方法可能是当前治疗选择中的有益补充。
