Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330031, PR China.
Biochem Biophys Res Commun. 2011 Apr 8;407(2):301-6. doi: 10.1016/j.bbrc.2011.03.006. Epub 2011 Mar 5.
Soluble CD4 (sCD4), anti-CD4 antibody, and anti-gp120 antibody have long been regarded as entry inhibitors in human immunodeficiency virus (HIV) therapy. However, the interactions between these HIV entry inhibitors and corresponding target molecules are still poorly understood. In this study, atomic force microscopy (AFM) was utilized to investigate the interaction forces among them. We found that the unbinding forces of sCD4-gp120 interaction, CD4 antigen-antibody interaction, and gp120 antigen-antibody interaction were 25.45 ± 20.46, 51.2 2 ± 34.64, and 89.87 ± 44.63 pN, respectively, which may provide important mechanical information for understanding the effects of viral entry inhibitors on HIV infection. Moreover, we found that the functionalization of an interaction pair on AFM tip or substrate significantly influenced the results, implying that we must perform AFM force measurement and analyze the data with more caution.
可溶性 CD4（sCD4）、抗 CD4 抗体和抗 gp120 抗体长期以来一直被认为是人类免疫缺陷病毒（HIV）治疗中的进入抑制剂。然而，这些 HIV 进入抑制剂与相应靶分子之间的相互作用仍知之甚少。在这项研究中，原子力显微镜（AFM）被用于研究它们之间的相互作用力。我们发现，sCD4-gp120 相互作用、CD4 抗原-抗体相互作用和 gp120 抗原-抗体相互作用的解结合力分别为 25.45 ± 20.46、51.22 ± 34.64 和 89.87 ± 44.63 pN，这可能为理解病毒进入抑制剂对 HIV 感染的影响提供重要的力学信息。此外，我们发现 AFM 针尖或基底上相互作用对的功能化显著影响了结果，这意味着我们必须更谨慎地进行 AFM 力测量和分析数据。
