Theodoridis Georgios, Gika Helen G, Wilson Ian D
Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Mass Spectrom Rev. 2011 Sep-Oct;30(5):884-906. doi: 10.1002/mas.20306. Epub 2011 Mar 7.
Metabonomics and metabolomics represent one of the three major platforms in systems biology. To perform metabolomics it is necessary to generate comprehensive "global" metabolite profiles from complex samples, for example, biological fluids or tissue extracts. Analytical technologies based on mass spectrometry (MS), and in particular on liquid chromatography-MS (LC-MS), have become a major tool providing a significant source of global metabolite profiling data. In the present review we describe and compare the utility of the different analytical strategies and technologies used for MS-based metabolomics with a particular focus on LC-MS. Both the advantages offered by the technology and also the challenges and limitations that need to be addressed for the successful application of LC-MS in metabolite analysis are described. Data treatment and approaches resulting in the detection and identification of biomarkers are considered. Special emphasis is given to validation issues, instrument stability, and QA/quality control (QC) procedures.
代谢组学是系统生物学的三大主要平台之一。要进行代谢组学研究，有必要从复杂样本（如生物体液或组织提取物）中生成全面的“全局”代谢物谱。基于质谱（MS），特别是液相色谱 - 质谱联用（LC - MS）的分析技术，已成为提供全局代谢物谱数据的重要来源的主要工具。在本综述中，我们描述并比较了用于基于质谱的代谢组学的不同分析策略和技术的效用，特别关注LC - MS。阐述了该技术的优势以及LC - MS在代谢物分析中成功应用需要解决的挑战和局限性。还考虑了导致生物标志物检测和鉴定的数据处理及方法。特别强调了验证问题、仪器稳定性以及质量保证/质量控制（QC）程序。
