Department of Aquaculture Biotechnology, Manonmaniam Sundaranar University, Sri Paramakalyani Centre for Environmental Sciences, Alwarkurchi, Tamil Nadu, India.
Pharm Biol. 2011 May;49(5):484-91. doi: 10.3109/13880209.2010.526615. Epub 2011 Mar 9.
Infectious diseases are one of the major factors affecting the production of fish worldwide. The pathogens (especially bacteria) affect the immune system of fish and the administration of immunostimulants can increase resistance to infectious diseases by enhancing both specific and nonspecific defense mechanisms.
In the present study, we have conducted an experiment on the pathogen-infected Cyprinus carpio Linn. (Cyprinidae), using Euphorbia hirta Linn. (Euphorbiaceae) plant leaves as immunostimulants.
The aqueous extract of the leaves was prepared and the immunostimulant action was recorded by giving different concentrations of plant extract supplemented diet.
The results obtained from the studies show that the higher concentration of the extract (50 g/kg diet) provided significant immune response (specific and nonspecific) on the fish. The 50 g/kg leaf extract of E. hirta enhanced the phagocytic ratio on 10th and 15th day after the infection. The results of the specific and nonspecific immunostimulation studies are statistically significant.
This work will guide the researchers for the discovery of significant aquaculture nutrients to improve the immunostimulant action on fish.
传染病是影响全球鱼类生产的主要因素之一。病原体（尤其是细菌）会影响鱼类的免疫系统，而免疫刺激剂的使用可以通过增强特异性和非特异性防御机制来提高对传染病的抵抗力。
本研究以大戟科(Euphorbiaceae)植物叶作为免疫刺激剂，对感染病原体的鲤鱼（鲤科）进行了实验。
制备了叶片的水提取物，并通过给予不同浓度的植物提取物补充饮食来记录免疫刺激作用。
研究结果表明，较高浓度的提取物（50g/kg 饮食）对鱼类提供了显著的免疫反应（特异性和非特异性）。大戟属植物叶 50g/kg 的提取物在感染后第 10 天和第 15 天提高了吞噬比率。特异性和非特异性免疫刺激研究的结果具有统计学意义。
这项工作将指导研究人员发现具有重要水产养殖营养价值的物质，以提高鱼类的免疫刺激作用。
