Department of Biomolecular Sciences, University of Urbino Carlo Bo, Urbino (PU), Italy.
Nanomedicine (Lond). 2011 Feb;6(2):211-23. doi: 10.2217/nnm.10.163.
The half-life of superparamagnetic iron oxide nanoparticles in the bloodstream is very short since they are rapidly taken up by the reticuloendothelial system. In this article, we report the encapsulation of different magnetic nanoparticles into human erythrocytes to increase their blood circulation time.
MATERIALS & METHODS: Newly synthesized and commercially available nanoparticles were evaluated for the encapsulation into red blood cells through the transient opening of membrane pores by controlled hypotonic dialysis and successive isotonic resealing and reannealing of cells.
Commercial superparamagnetic iron oxide nanoparticles (SHU 555A, AMI 227 and PMP-50) dextran or carboxydextran coated can be successfully loaded into red blood cells; similarly, some of the new nanomaterials, such as Np-1 nanoparticles dispersed in the Disperbyk®-190 agent, can be efficiently encapsulated into red blood cells.
A careful consideration of magnetic nanoparticles parameters, such as size, synthesis protocols, coating and/or dispersant agents, is required in order to obtain efficient loading through the cell membrane pores.
超顺磁性氧化铁纳米颗粒在血液中的半衰期非常短，因为它们很快被网状内皮系统吸收。本文报道了将不同的磁性纳米颗粒封装到人类红细胞中以增加其血液循环时间。
通过控制的低渗透析使细胞膜短暂打开孔，然后进行等渗再封闭和再退火，将新合成的和市售的纳米颗粒评估为封装到红细胞中。
商业超顺磁性氧化铁纳米颗粒（SHU 555A、AMI 227 和 PMP-50）葡聚糖或羧基葡聚糖涂层可以成功地装入红细胞中；同样，一些新的纳米材料，如分散在 Disperbyk®-190 试剂中的 Np-1 纳米颗粒，可以有效地封装到红细胞中。
为了通过细胞膜孔实现有效的负载，需要仔细考虑磁性纳米颗粒的参数，如尺寸、合成方案、涂层和/或分散剂。
