Department of Biomolecular Sciences, University of Urbino Carlo Bo, Via Saffi 2, 61029, Urbino (PU), Italy.
Section for Biomedical Imaging, University Medical Center Hamburg-Eppendorf, Lottestr. 55, 22529, Hamburg, Germany.
Nanomedicine (Lond). 2020 Apr;15(8):739-753. doi: 10.2217/nnm-2019-0449. Epub 2020 Mar 24.
Magnetic particle imaging (MPI) is highly promising for biomedical applications, but optimal tracers for MPI, namely superparamagnetic iron oxide-based contrast agents, are still lacking. The encapsulation of commercially available nanoparticles, specifically synomag-D and perimag, into human red blood cells (RBCs) was performed by a hypotonic dialysis and isotonic resealing procedure. The amounts of superparamagnetic iron oxide incorporated into RBCs were determined by Fe quantification using nuclear magnetic resonance and magnetic particle spectroscopy. Perimag-COOH nanoparticles were identified as the best nanomaterial for encapsulation in RBCs. Perimag-COOH-loaded RBCs proved to be viable cells showing a good magnetic particle spectroscopy performance, while the magnetic signal of synomag-D-COOH-loaded RBCs dropped sharply. Perimag-COOH-loaded RBCs could be a potential tool for MPI diagnostic applications.
磁粒子成像（MPI）在生物医学应用中具有广阔的前景，但 MPI 的最佳示踪剂，即超顺磁性氧化铁基对比剂，仍然缺乏。通过低渗透析和等渗再封闭的程序，将市售的纳米颗粒（特别是 synomag-D 和 perimag）封装到人类红细胞（RBC）中。通过核磁共振和磁粒子光谱法对铁的定量分析，确定了超顺磁性氧化铁在 RBC 中的掺入量。perimag-COOH 纳米颗粒被确定为 RBC 包封的最佳纳米材料。证明负载 perimag-COOH 的 RBC 是可行的细胞，具有良好的磁粒子光谱性能，而负载 synomag-D-COOH 的 RBC 的磁信号急剧下降。负载 perimag-COOH 的 RBC 可能是 MPI 诊断应用的潜在工具。
