Rajawat Jyotika, Mir Hina, Begum Rasheedunnisa
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara-390002, Gujarat, India.
BMC Dev Biol. 2011 Mar 9;11:14. doi: 10.1186/1471-213X-11-14.
Poly(ADP-ribose) polymerase is evolutionarily conserved as a responder to various forms of stress. Though PARP's role in cell death is well addressed, its role in development and multicellularity is still an enigma. We have previously reported the role of PARP in oxidative stress induced delayed development of D. discoideum.
In the current study we highlight the involvement of PARP during D. discoideum development. Oxidative stress affects expression of aca and cAR1 thus affecting aggregation. Although parp expression is not affected during oxidative stress but it is involved during normal development as confirmed by our PARP down-regulation studies. Constitutive PARP down-regulation resulted in blocked development while no effect was observed on D. discoideum growth. Interestingly, stage specific PARP down-regulation arrested development at the slug stage.
These results emphasize that PARP is essential for complex differentiation and its function may be linked to multicellularity. This is the first report where the involvement of PARP during normal multicellular development in D. discoideum, an ancient eukaryote, is established which could be of evolutionary significance. Thus our study adds one more role to the multitasking function of PARP.
聚（ADP - 核糖）聚合酶在进化过程中作为对各种应激形式的响应者而保守存在。尽管PARP在细胞死亡中的作用已得到充分研究，但其在发育和多细胞性中的作用仍是一个谜。我们之前报道过PARP在氧化应激诱导的盘基网柄菌发育延迟中的作用。
在当前研究中，我们强调了PARP在盘基网柄菌发育过程中的参与情况。氧化应激影响aca和cAR1的表达，从而影响聚集。虽然在氧化应激期间parp表达不受影响，但我们的PARP下调研究证实它在正常发育过程中发挥作用。组成型PARP下调导致发育受阻，而对盘基网柄菌的生长没有影响。有趣的是，阶段特异性PARP下调使发育停滞在蛞蝓阶段。
这些结果强调PARP对于复杂分化至关重要，其功能可能与多细胞性相关。这是首次报道PARP在古老真核生物盘基网柄菌正常多细胞发育过程中的参与情况，这可能具有进化意义。因此，我们的研究为PARP的多任务功能增添了又一个作用。
