Department of Reproduction and Developmental Biology, Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovakia.
J Proteomics. 2011 Aug 12;74(8):1378-84. doi: 10.1016/j.jprot.2011.02.029. Epub 2011 Mar 6.
Molecular characterization of crop plants grown in remediated, formerly radioactive, areas could establish a framework for future agricultural use of these areas. Recently, we have established a quantitative reference map for mature flax seed proteins (Linum usitatissimum L.) harvested from a remediated plot in Chernobyl town. Herein we describe results from our ongoing studies of this subject, and provide a proteomics-based characterization of developing flax seeds harvested from same field. A quantitative approach, based on 2-dimensional electrophoresis (2-DE) and tandem mass spectrometry, yielded expression profiles for 379 2-DE spots through seed development. Despite the paucity of genomic resources for flax, the identity for 102 proteins was reliably determined. These proteins were sorted into 11 metabolic functional classes. Proteins of unknown function comprise the largest group, and displayed a pattern of decreased abundance throughout seed development. Analysis of the composite expression profiles for metabolic protein classes revealed specific expression patterns during seed development. For example, there was an overall decrease in abundance of the glycolytic enzymes during seed development.
在经过修复的、曾经有放射性的地区种植的农作物的分子特征，可以为未来这些地区的农业利用建立一个框架。最近，我们已经为切尔诺贝利镇一个修复区收获的成熟亚麻籽蛋白质（Linum usitatissimum L.）建立了一个定量参考图谱。在此，我们描述了我们正在进行的这一主题研究的结果，并提供了从同一田间收获的发育中的亚麻籽的基于蛋白质组学的特征描述。通过种子发育，基于二维电泳（2-DE）和串联质谱的定量方法产生了 379 个 2-DE 斑点的表达谱。尽管亚麻基因组资源匮乏，但 102 种蛋白质的身份得到了可靠确定。这些蛋白质被分为 11 种代谢功能类别。具有未知功能的蛋白质构成了最大的组，并在整个种子发育过程中表现出丰度降低的模式。对代谢蛋白类别的综合表达谱进行分析，揭示了种子发育过程中的特定表达模式。例如，在种子发育过程中，糖酵解酶的丰度总体上呈下降趋势。
