College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, United States of America.
PLoS One. 2011 Feb 28;6(2):e17111. doi: 10.1371/journal.pone.0017111.
Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium able to utilize CO and H(2) to derive energy for fixation of CO(2). Thus, it is capable of growth using syngas, which is a mixture of varying amounts of CO and H(2) generated by organic waste gasification. O. carboxidovorans is capable also of heterotrophic growth in standard bacteriologic media. Here we characterize how the O. carboxidovorans proteome adapts to different lifestyles of chemolithoautotrophy and heterotrophy. Fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown with acetate or with syngas showed that the bacterium changes membrane fatty acid composition. Quantitative shotgun proteomic analysis of O. carboxidovorans grown in the presence of acetate and syngas showed production of proteins encoded on the megaplasmid for assimilating CO and H(2) as well as proteins encoded on the chromosome that might have contributed to fatty acid and acetate metabolism. We found that adaptation to chemolithoautotrophic growth involved adaptations in cell envelope, oxidative homeostasis, and metabolic pathways such as glyoxylate shunt and amino acid/cofactor biosynthetic enzymes.
寡养单胞菌 OM5 T.（DSM 1227，ATCC 49405）是一种化能自养细菌，能够利用 CO 和 H(2) 为 CO(2) 的固定提供能量。因此，它能够使用合成气（有机废气气化产生的 CO 和 H(2) 的混合物）进行生长。寡养单胞菌也能够在标准细菌培养基中进行异养生长。在这里，我们描述了寡养单胞菌的蛋白质组如何适应化能自养和异养的不同生活方式。用乙酸或合成气培养的寡养单胞菌的脂肪酸甲酯 (FAME) 分析表明，细菌改变了膜脂肪酸组成。用乙酸和合成气培养的寡养单胞菌的定量 shotgun 蛋白质组学分析表明，产生了用于同化 CO 和 H(2) 的巨质粒上编码的蛋白质以及可能有助于脂肪酸和乙酸代谢的染色体上编码的蛋白质。我们发现，适应化能自养生长涉及细胞包膜、氧化平衡和代谢途径（如乙醛酸支路和氨基酸/辅酶生物合成酶）的适应性变化。
