Microbial Biotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany.
SynBiofoundry@TUM, Technical University of Munich, Straubing, Germany.
Adv Biochem Eng Biotechnol. 2022;180:1-32. doi: 10.1007/10_2021_178.
Aerobic carboxydotrophic bacteria are a group of microorganisms which possess the unique trait to oxidize carbon monoxide (CO) as sole energy source with molecular oxygen (O) to produce carbon dioxide (CO) which subsequently is used for biomass formation via the Calvin-Benson-Bassham cycle. Moreover, most carboxydotrophs are also able to oxidize hydrogen (H) with hydrogenases to drive the reduction of carbon dioxide in the absence of CO. As several abundant industrial off-gases contain significant amounts of CO, CO, H as well as O, these bacteria come into focus for industrial application to produce chemicals and fuels from such gases in gas fermentation approaches. Since the group of carboxydotrophic bacteria is rather unknown and not very well investigated, we will provide an overview about their lifestyle and the underlying metabolic characteristics, introduce promising members for industrial application, and give an overview of available genetic engineering tools. We will point to limitations and discuss challenges, which have to be overcome to apply metabolic engineering approaches and to utilize aerobic carboxydotrophs in the industrial environment.
好氧羧化营养细菌是一群具有独特特性的微生物，它们可以将一氧化碳（CO）氧化为唯一的能量来源，同时利用分子氧（O）将其转化为二氧化碳（CO），随后通过卡尔文-本森-巴斯汉姆循环将其用于生物量的形成。此外，大多数羧化营养菌还能够利用氢化酶将氢气（H）氧化，从而在没有 CO 的情况下驱动二氧化碳的还原。由于几种丰富的工业废气中含有大量的 CO、CO、H 和 O，这些细菌成为了工业应用的焦点，可用于通过气体发酵方法从这些气体中生产化学品和燃料。由于羧化营养菌的群体相对未知，研究也不是很充分，因此，我们将概述它们的生活方式和潜在的代谢特征，介绍有应用前景的成员，并概述现有的遗传工程工具。我们将指出限制并讨论挑战，这些挑战必须克服，才能应用代谢工程方法，并在工业环境中利用好氧羧化营养菌。
