College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, USA.
BMC Genomics. 2010 Sep 23;11:511. doi: 10.1186/1471-2164-11-511.
Oligotropha carboxidovorans OM5 T. (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium capable of utilizing CO (carbon monoxide) and fixing CO2 (carbon dioxide). We previously published the draft genome of this organism and recently submitted the complete genome sequence to GenBank.
The genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5 consists of a 3.74-Mb chromosome and a 133-kb megaplasmid that contains the genes responsible for utilization of carbon monoxide, carbon dioxide, and hydrogen. To our knowledge, this strain is the first one to be sequenced in the genus Oligotropha, the closest fully sequenced relatives being Bradyrhizobium sp. BTAi and USDA110 and Nitrobacter hamburgiensis X14. Analysis of the O. carboxidovorans genome reveals potential links between plasmid-encoded chemolithoautotrophy and chromosomally-encoded lipid metabolism. Comparative analysis of O. carboxidovorans with closely related species revealed differences in metabolic pathways, particularly in carbohydrate and lipid metabolism, as well as transport pathways.
Oligotropha, Bradyrhizobium sp and Nitrobacter hamburgiensis X14 are phylogenetically proximal. Although there is significant conservation of genome organization between the species, there are major differences in many metabolic pathways that reflect the adaptive strategies unique to each species.
寡养单胞菌 OM5 T.（DSM 1227，ATCC 49405）是一种能够利用 CO（一氧化碳）和固定 CO2（二氧化碳）的化能自养细菌。我们之前发表了该生物的基因组草案，并最近向 GenBank 提交了完整的基因组序列。
化能自养细菌寡养单胞菌 OM5 的基因组序列由一个 3.74-Mb 染色体和一个 133-kb 大型质粒组成，其中包含负责利用一氧化碳、二氧化碳和氢气的基因。据我们所知，该菌株是寡养单胞菌属中第一个被测序的菌株，与其亲缘关系最近的全序列菌株是慢生根瘤菌 BTAi 和 USDA110 以及硝化杆菌 X14。寡养单胞菌 OM5 基因组的分析揭示了质粒编码的化能自养与染色体编码的脂质代谢之间的潜在联系。与密切相关的物种进行的比较分析显示，代谢途径存在差异，特别是在碳水化合物和脂质代谢以及运输途径方面。
寡养单胞菌、慢生根瘤菌和硝化杆菌 X14 在系统发育上较为接近。尽管物种之间的基因组组织具有显著的保守性，但许多代谢途径存在重大差异，反映了每个物种特有的适应策略。
