巴西马托格罗索州屠宰猪中产肠毒素和产志贺毒素大肠杆菌的流行情况。
Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil.
作者信息
Martins Rodrigo Prado, da Silva Maria Cristina, Dutra Valeria, Nakazato Luciano, Leite Domingos da Silva
机构信息
Departamento de Clínica Médica Veterinária, Universidade Federal de Mato Grosso, Brazil.
出版信息
J Infect Dev Ctries. 2011 Mar 2;5(2):123-7. doi: 10.3855/jidc.1217.
INTRODUCTION
This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil.
METHODOLOGY
Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays.
RESULTS
It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1.
CONCLUSION
The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso.
引言
本研究旨在估计巴西马托格罗索州屠宰场宰杀的猪中肠毒素性大肠杆菌（ETEC）和产志贺毒素大肠杆菌（STEC）菌株的流行率。
方法
对74只动物的肠道样本进行无菌解剖，并将肠腔内容物接种于麦康凯琼脂平板上。通过PCR检测对每个平板上的融合菌落进行ETEC和STEC菌株筛查。
结果
经证实，STEC和ETEC携带率分别为1.35%和9.46%。在74份检测样本中，1份（1.35%）stx2基因呈阳性，7份（9.46%）st1基因呈阳性，其中2份（2.70%）lt1基因也呈阳性。
结论
所提供的结果为马托格罗索州猪源致病性大肠杆菌的未来研究提供了一个基准。
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