Department of Veterinary Clinics, University of Pisa, Pisa, Italy.
J Pediatr. 2011 Aug;159(2):278-83.e1. doi: 10.1016/j.jpeds.2011.01.028. Epub 2011 Mar 10.
To determine the concentrations of zearalenone and its metabolites in the leading brands of infant formula milks and meat-based infant foods commonly marketed in Italy, and to assess their repercussion in the provisional tolerable daily intakes of these estrogenic mycotoxins.
A total of 185 cow's milk-based infant formulas and 44 samples of meat-based infant foods samples were analyzed. The analysis of mycotoxins was performed by immunoaffinity column clean-up and high-pressure liquid chromatography with fluorescence detection.
Zearalenone was detected in 17 (9%) milk samples (maximum 0.76 μg/L). The α-zearalenol was detected in 49 (26%) milk samples (maximum 12.91 μg/L). The β-zearalenol was detected in 53 (28%) milk samples (maximum 73.24 μg/L). The α-zearalanol and β-zearalanol were not detected in milk samples. Although α-zearalenol was detected in 12 (27%) meat samples (maximum 30.50 μg/kg), only one meat-based sample was contaminated by α-zearalanol (950 μg/kg). Zearalenone, β-zearalenol, and β-zearalanol were not detected in meat samples.
This study shows the presence of mycoestrogens in infant (milk-based and meat-based) food, and this is likely to have great implications for subsequent generations, suggesting the need to perform occurrence surveys in this type of food.
测定意大利市场上常见的领先品牌婴儿配方奶和以肉类为基础的婴儿食品中玉米赤霉烯酮及其代谢物的浓度，并评估这些雌激素霉菌毒素在暂定可耐受每日摄入量中的影响。
共分析了 185 种牛奶基婴儿配方奶粉和 44 种以肉类为基础的婴儿食品样本。采用免疫亲和柱净化和高效液相色谱荧光检测法进行霉菌毒素分析。
在 17 种（9%）牛奶样本中检测到玉米赤霉烯酮（最高 0.76μg/L）。在 49 种（26%）牛奶样本中检测到α-玉米赤醇（最高 12.91μg/L）。在 53 种（28%）牛奶样本中检测到β-玉米赤醇（最高 73.24μg/L）。在牛奶样本中未检测到α-玉米赤烯醇和β-玉米赤烯醇。虽然在 12 种（27%）肉类样本中检测到α-玉米赤醇（最高 30.50μg/kg），但只有一种以肉类为基础的样本被α-玉米赤烯醇污染（950μg/kg）。在肉类样本中未检测到玉米赤霉烯酮、β-玉米赤醇和β-玉米赤烯醇。
本研究表明，婴儿（牛奶基和以肉类为基础的）食品中存在霉菌雌激素，这可能对后代产生重大影响，表明需要对这类食品进行出现情况调查。
