Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2011 Apr 15;26(8):3714-8. doi: 10.1016/j.bios.2011.02.024. Epub 2011 Feb 21.
Nanoporous gold (NPG) film modified electrode for the construction of novel label-free electrochemical immunosensor for ultrasensitive detection of cancer biomarker prostate specific antigen (PSA) is described. Due to its high conductivity, large surface area, and good biocompatibility, NPG film modified electrode was used for the adsorption of anti-PSA antibody (Ab). The sensing signal is based on the monitoring of the electrode's current response towards K(3)Fe(CN)(6), which is extremely sensitive to the formation of immunocomplex within the nanoporous film. Under optimum conditions, the amperometric signal decreases linearly with PSA concentration (0.05-26 ng/mL), resulting in a low limit of detection (3 pg/mL). We demonstrated the application of the novel immunosensor for the detection of PSA in real sample with satisfactory results.
纳米多孔金（NPG）修饰电极用于构建新型无标记电化学免疫传感器，用于超灵敏检测癌症生物标志物前列腺特异性抗原（PSA）。由于其高导电性、大表面积和良好的生物相容性，NPG 修饰电极被用于吸附抗 PSA 抗体（Ab）。传感信号基于监测电极对 K（3）Fe（CN）（6）的电流响应，K（3）Fe（CN）（6）对纳米多孔膜内免疫复合物的形成非常敏感。在最佳条件下，电流响应与 PSA 浓度（0.05-26ng/mL）呈线性关系，检测限低至 3pg/mL。我们展示了该新型免疫传感器在实际样品中检测 PSA 的应用，结果令人满意。
