Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, People's Republic of China.
Anal Biochem. 2012 Mar 1;422(1):22-7. doi: 10.1016/j.ab.2011.12.047. Epub 2012 Jan 8.
For the specificity of prostate cancer markers, prostate specific antigen (PSA) has been widely used in prostate cancer screening, diagnosis, and treatment after monitoring. In normal male serum, PSA can only be detected in traces of 0-4 ng mL(-1). In this paper, we constructed an electrochemical immunosensor for PSA detection using a nanocomposite film of graphene sheets-methylene blue-chitosan (GS-MB-CS) as electrode material. The nanocomposite film showed high binding affinity to the electrode and was used to immobilize the antibody of PSA. The modification procedure was monitored by cyclic voltammetry (CV). An amperometric biosensor was easily developed based on the response of peak current to the capture of PSA induced by specific antigen-antibody reactions. Under optimum conditions, the amperometric signal decreased linearly with PSA concentration (0.05-5.00 ng mL(-1)). A low limit of detection (13 pg mL(-1)) and a high selectivity are obtained. Moreover, the prepared immunosensor was applied for the analysis of PSA in serum samples with satisfactory results. The proposed method may have a promising future in biochemical assays for high selectivity, good reproducibility, and stability.
由于前列腺癌标志物的特异性，前列腺特异性抗原（PSA）已被广泛用于前列腺癌的筛查、诊断和监测后的治疗。在正常男性血清中，PSA 只能在痕量的 0-4ngmL(-1) 中检测到。在本文中，我们使用石墨烯片-亚甲蓝-壳聚糖（GS-MB-CS）纳米复合材料膜作为电极材料，构建了用于 PSA 检测的电化学免疫传感器。纳米复合材料膜与电极具有高结合亲和力，并用于固定 PSA 的抗体。修饰过程通过循环伏安法（CV）进行监测。基于特异性抗原-抗体反应引起的 PSA 捕获对峰电流的响应，很容易开发出安培生物传感器。在最佳条件下，安培信号随 PSA 浓度（0.05-5.00ngmL(-1)）呈线性下降。获得了低检测限（13pgmL(-1)）和高选择性。此外，所制备的免疫传感器用于血清样品中 PSA 的分析，结果令人满意。该方法在具有高选择性、良好重现性和稳定性的生化分析中可能具有广阔的前景。
