Abbott Medical Optics Inc., Santa Ana, CA 92705-4933, USA.
Cont Lens Anterior Eye. 2011 Aug;34(4):183-7. doi: 10.1016/j.clae.2011.02.010. Epub 2011 Mar 9.
Non-compliance is a significant factor in contact lens related microbial keratitis and includes solution reuse and failure to recap the lens storage case resulting in evaporation effects. To address this, impact of partial evaporation on the antimicrobial efficacy of multipurpose contact lens care solutions was investigated.
Solutions were evaporated under a stream of air to 2× and 4× concentration and challenged with Fusarium solani (ATCC 36031), Candida albicans (ATCC 10231) and Acanthamoeba castellanii (ATCC 50370). The level of organism kill at 6h was compared to the non-evaporated product.
ReNu with MoistureLoc(®) (RML) lost 90-100% of biocidal activity against C. albicans on evaporation, 75-99% for F. solani and 29-33% with A. castellanii at 2× or 4× concentration, respectively. OPTI-FREE(®) RepleniSH(®) lost 72-90% efficacy against C. albicans and F. solani, and 61% at 2× and 10% at 4× concentration with A. castellanii. ReNu(®) MultiPlus, AQuify(®) Multi-Purpose and Biotrue™ showed only loss in efficacy with C. albicans at 4× concentration giving 79%, 34.5% and 48% reduction, respectively. No loss in biocidal activity on evaporation was obtained with Complete(®) Revitalens for all organisms.
Partial evaporation can affect biocidal efficacy of multi-purpose solutions and may have been a significant factor in an outbreak of Fusarium keratitis cases associated with RML. Evaporation results in increased binding of cationic disinfectants to counter-ions in the formulation, reducing ability to attach and rupture anionic microbial cell walls. Interaction may also occur between the biocidal ingredient and other components, such as surfactants, resulting in sequestration of activity through micelle formation.
不遵守规定是接触镜相关微生物角膜炎的一个重要因素，包括溶液的重复使用以及未能重新盖上隐形眼镜储存盒导致蒸发效应。为了解决这个问题，研究了部分蒸发对多功能隐形眼镜护理液的抗菌功效的影响。
将溶液在空气流下蒸发至 2 倍和 4 倍浓度，并分别用茄病镰刀菌（ATCC 36031）、白色念珠菌（ATCC 10231）和棘阿米巴（ATCC 50370）进行挑战。在 6 小时时比较了与未蒸发产品的杀菌水平。
ReNu with MoistureLoc®（RML）在蒸发时对白色念珠菌的杀菌活性丧失了 90-100%，对茄病镰刀菌的杀菌活性丧失了 75-99%，对棘阿米巴的杀菌活性丧失了 29-33%，浓度分别为 2 倍或 4 倍。OPTIFREE® RepleniSH®对白色念珠菌和茄病镰刀菌的功效丧失了 72-90%，对棘阿米巴的功效丧失了 61%，浓度为 2 倍，丧失了 10%，浓度为 4 倍。ReNu® MultiPlus、AQuify® Multi-Purpose 和 Biotrue™ 仅在 4 倍浓度下对白色念珠菌的功效降低，分别为 79%、34.5%和 48%。对于所有的微生物，Complete® Revitalens 在蒸发时没有杀菌活性的丧失。
部分蒸发会影响多用途溶液的杀菌功效，并且可能是与 RML 相关的茄病镰刀菌角膜炎爆发的一个重要因素。蒸发会导致阳离子消毒剂与配方中的反离子结合增加，从而降低其附着和破坏阴离子微生物细胞壁的能力。杀菌成分与其他成分（如表面活性剂）之间也可能发生相互作用，导致通过胶束形成使活性被隔离。
