Biology Department, Georgia State University, Atlanta, Georgia, USA.
Invest Ophthalmol Vis Sci. 2011 Mar 28;52(3):1793-9. doi: 10.1167/iovs.10-5956.
To examine in vitro effects of evaporation and drying of multipurpose contact lens solutions on survival of Fusarium and Acanthamoeba.
Conidia of representative Fusarium from the 2004-2006 keratitis outbreak and trophozoites of Acanthamoeba castellanii were inoculated into commercially available multipurpose contact lens care solutions. These solutions were inoculated with 10(2)-10(6) microbial propagules/mL and were evaporated for at least 24 hours. After drying, nutrient media for recovery of surviving organisms were added to the residues formed in the lids of 38 mm polystyrene Petri dishes. General morphologic patterns of the solution residuals and the distribution and morphologies of the microorganisms were recorded with microscopic imaging.
Various multipurpose contact lens disinfection solutions formed distinctive dried residual patterns. Both Fusarium and Acanthamoeba at concentrations tested above 10(3) per mL of disinfection solution were recovered from dried films with replicate testing. Mature cysts of Acanthamoeba not evident in the inocula were observed in sparse numbers in all dried solutions except one (Complete Moisture Plus; Advanced Medical Optics) and control salines where precysts and mature cysts were common. Both fusaria and amoeba tended to be observed in discrete regions of the dried residues.
Regions of drying films of multipurpose contact lens disinfection solutions on contact lens cases may induce and harbor dormant-resistant stages of Fusarium and Acanthamoeba. It is hypothesized that the evaporation and drying of multipurpose contact lens disinfection solutions may have been an added risk factor for case contamination among Fusarium and Acanthamoeba keratitis patients. The need for frequent replacement of contact lens cases is enforced.
研究多用途隐形眼镜护理液蒸发和干燥对镰刀菌和棘阿米巴存活的体外影响。
将来自 2004-2006 年角膜炎爆发的代表性镰刀菌的分生孢子和棘阿米巴包囊接种到市售的多用途隐形眼镜护理液中。这些溶液接种了 10（2）-10（6）个微生物繁殖体/ml，并至少蒸发 24 小时。干燥后，向 38mm 聚苯乙烯培养皿盖内形成的残留物中添加用于回收存活生物的营养培养基。用显微镜成像记录溶液残留物的一般形态模式以及微生物的分布和形态。
各种多用途隐形眼镜消毒溶液形成了独特的干燥残留模式。在测试浓度高于 10（3）/ml 消毒溶液中，均从干燥薄膜中重复测试回收了镰刀菌和棘阿米巴。在所有干燥溶液中，除一种（Complete Moisture Plus；Advanced Medical Optics）和对照盐水中未明显出现接种物中的成熟包囊外，观察到稀疏数量的成熟棘阿米巴包囊。在干燥残留物的离散区域中均观察到两种镰刀菌和阿米巴。
隐形眼镜盒上多用途隐形眼镜消毒溶液干燥膜的区域可能诱导和藏匿休眠抗性的镰刀菌和棘阿米巴阶段。据推测，多用途隐形眼镜消毒溶液的蒸发和干燥可能是导致镰刀菌和棘阿米巴角膜炎患者病例污染的一个附加风险因素。需要频繁更换隐形眼镜盒。
