The natriuretic and diuretic effects of atriopeptin III (125, 250 and 500 ng kg-1, i.v.) were studied in groups of rats anaesthetized with pentobarbitone which were either sham controls, unilaterally nephrectomized controls, adenine-fed or subtotal nephrectomy chronic renal failure models. 2. Atriopeptin III given at these doses to the sham control animals had no effect on blood pressure, renal blood flow or glomerular filtration rate but reversibly increased urine flow, between 46% to 54%, absolute sodium excretion, between 52% to 61%, and fractional sodium excretion, between 48% to 54% (all P values less than 0.05) from the lowest to the highest dose. The adenine-fed chronic renal failure group of rats had a reduced renal blood flow of between 30 and 75%, and glomerular filtration rate of approximately 20%, compared to the sham controls. Administration of atriopeptin at 125, 250 and 500 ng kg-1 to the animals with renal failure increased water and sodium excretion to the same degree as observed in the sham group of rats. 3. In the group of unilaterally nephrectomized rats, atriopeptin III, at 125, 250 and 500 ng kg-1 increased urine flow by 36%, 47% and 72%, respectively, absolute sodium excretion by 37%, 57% and 106%, respectively, and fractional sodium excretion by 46%, 45% and 102%, respectively. A similar pattern of responses was observed in the subtotal nephrectomy, chronic renal failure group in which filtration rate was approximately 4 times less than the controls. 4. These results show that in two different models of chronic renal failure, atriopeptin III still caused a natriuresis and diuresis. This suggests that the nephrons retain sensitivity to the atrial natriuretic peptides in diseases such as chronic renal failure and that these compounds may be useful in mobilizing body fluids in this situation.
摘要
研究了心房肽III（125、250和500 ng kg-1，静脉注射）对戊巴比妥麻醉的大鼠组的利钠和利尿作用，这些大鼠组包括假手术对照组、单侧肾切除对照组、腺嘌呤喂养组或次全肾切除慢性肾衰竭模型组。2. 给假手术对照动物注射这些剂量的心房肽III对血压、肾血流量或肾小球滤过率没有影响，但从最低剂量到最高剂量，可使尿量可逆性增加46%至54%，绝对钠排泄量增加52%至61%，钠排泄分数增加48%至54%（所有P值均小于0.05）。与假手术对照组相比，腺嘌呤喂养的慢性肾衰竭大鼠组肾血流量减少30%至75%，肾小球滤过率约降低20%。给肾衰竭动物注射125、250和500 ng kg-1的心房肽，水和钠排泄增加的程度与假手术大鼠组相同。3. 在单侧肾切除大鼠组中，125、250和500 ng kg-1的心房肽III分别使尿量增加36%、47%和72%，绝对钠排泄量分别增加37%、57%和106%，钠排泄分数分别增加46%、45%和102%。在次全肾切除慢性肾衰竭组中观察到类似的反应模式，其滤过率比对照组低约4倍。4. 这些结果表明，在两种不同的慢性肾衰竭模型中，心房肽III仍能引起利钠和利尿作用。这表明在慢性肾衰竭等疾病中，肾单位对心房利钠肽仍保持敏感性，并且这些化合物在这种情况下可能有助于动员体液。
