Centre for Genomic Regulation (CRG), Barcelona, Spain.
Science. 2011 Apr 22;332(6028):465-8. doi: 10.1126/science.1201578. Epub 2011 Mar 10.
Partitioning of chromatids during mitosis requires that chromosome compaction and spindle length scale appropriately with each other. However, it is not clear whether chromosome condensation and spindle elongation are linked. Here, we find that yeast cells could cope with a 45% increase in the length of their longest chromosome arm by increasing its condensation. The spindle midzone, aurora/Ipl1 activity, and Ser10 of histone H3 mediated this response. Thus, the anaphase spindle may function as a ruler to adapt the condensation of chromatids, promoting their segregation regardless of chromosome or spindle length.
有丝分裂过程中染色体的分离要求染色体的压缩和纺锤体的长度彼此之间要适当缩放。然而，染色体的浓缩和纺锤体的伸长是否相关还不清楚。在这里，我们发现酵母细胞可以通过增加其最长染色体臂的浓缩程度来应对其长度增加 45%的情况。纺锤体中部区域、极光激酶/Ipl1 活性和组蛋白 H3 的 Ser10 介导了这一反应。因此，后期纺锤体可能充当尺子来调节染色单体的浓缩，促进其分离，而与染色体或纺锤体长度无关。
