利用H₂O/D₂O同位素置换对淀粉样蛋白进行中子纤维衍射研究。
Neutron fibre diffraction studies of amyloid using H2O/D2O isotopic replacement.
作者信息
Tiggelaar Sarah M, Mossou Estelle, Callow Phil, Callow Shirley, Teixeira Susana C M, Mitchell Edward P, Mitraki Anna, Forsyth V Trevor
机构信息
Partnership for Structural Biology, Institut Laue-Langevin, France.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):332-5. doi: 10.1107/S1744309111002351. Epub 2011 Feb 23.
Abstract
The first neutron fibre diffraction studies of an amyloid system are presented. The techniques used to prepare the large samples needed are described, as well as the procedures used to isotopically replace H2O in the sample by D2O. The results demonstrate the feasibility of this type of approach for the pursuit of novel structural analyses that will strongly complement X-ray fibre diffraction studies and probe aspects of amyloid structure that to date have remained obscure. The approach is demonstrated using an amyloid form of the peptide NSGAITIG, but is equally applicable for the study of other systems such as Alzheimer's Aβ peptide.
摘要
首次展示了对淀粉样蛋白系统的中子纤维衍射研究。描述了制备所需大样本所使用的技术，以及用重水（D2O）对样本中的水（H2O）进行同位素置换的程序。结果表明，这种方法对于开展新型结构分析是可行的，这将有力地补充X射线纤维衍射研究，并探究迄今仍不清楚的淀粉样蛋白结构方面。该方法通过肽NSGAITIG的一种淀粉样形式得到验证，但同样适用于其他系统的研究，如阿尔茨海默病的Aβ肽。
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