Morris Kyle L, Serpell Louise C
School of Life Sciences, University of Sussex, Brighton, UK.
Methods Mol Biol. 2012;849:121-35. doi: 10.1007/978-1-61779-551-0_9.
Amyloid fibrils are polymeric assemblies of normally soluble proteins or peptides. To investigate their structure, it is generally not possible to use conventional methods of crystallography and solution nuclear magnetic resonance. To examine the repeating crystalline structure along the fibre axis, X-ray fibre diffraction has been a useful tool. Here we discuss the methods by which amyloid-like fibrils may be prepared to form a sample suitable for structural analysis and describe how data may be collected and then analysed to arrive at a potential model structure.
淀粉样纤维是通常可溶的蛋白质或肽的聚合物聚集体。为了研究它们的结构，一般无法使用传统的晶体学和溶液核磁共振方法。为了研究沿纤维轴的重复晶体结构，X射线纤维衍射一直是一种有用的工具。在这里，我们讨论了制备适合结构分析的淀粉样纤维样样品的方法，并描述了如何收集和分析数据以得出潜在的模型结构。
