Department of Orthopaedic Surgery, University of Virginia, Charlottesville, Virginia 22908, USA.
J Biomed Mater Res B Appl Biomater. 2011 May;97(2):299-305. doi: 10.1002/jbm.b.31815. Epub 2011 Mar 10.
The most successful metal implant materials currently have relatively smooth surfaces on the micron size scale, with most failures occurring after only 10 years. To move beyond this limiting time scale, texturing methods have been developed to modify the metal surface to enhance integration of the implant directly with surrounding bone. A flexible single-step ultrafast-laser texturing process has been developed that results in a surface texture that exhibits micron scale peaks and troughs with superimposed submicron and nano-scale features. The textured titanium samples remain completely hydrophilic with no measurable contact angle even after several weeks in normal atmosphere. An increase in mesenchymal stem cell number is observed over that on an untreated control titanium surface. Extensive formation of cellular bridges by stromal cells between pillars shows the favorable response of differentiated cells to the surface and the promotion of their attachment. Expression of the alkaline phosphatase and osteocalcin genes in human bone marrow cells were seen to increase on the textured surface. The development of this single-step method for creating micron, submicron, and nano-scale surface texture directly on metals makes a significant contribution to the goal of improving the integration and life span of joint replacement implants.
目前最成功的金属植入材料在微米尺度上具有相对光滑的表面，大多数失效发生在仅仅 10 年后。为了超越这个限制时间尺度，已经开发出织构方法来改变金属表面，以增强植入物与周围骨骼的直接整合。已经开发出一种灵活的单步超快激光织构工艺，该工艺导致表面纹理呈现出具有微米级峰谷的结构，并且具有叠加的亚微米和纳米级特征。即使在正常气氛下放置数周后，经织构处理的钛样品仍然保持完全亲水，没有可测量的接触角。与未经处理的对照钛表面相比，间充质干细胞的数量增加。基质细胞在柱子之间形成细胞桥的广泛形成表明分化细胞对表面的有利反应，并促进其附着。在经织构处理的表面上观察到人骨髓细胞中碱性磷酸酶和骨钙素基因的表达增加。这种直接在金属上制造微米、亚微米和纳米级表面结构的单步方法的开发，为提高关节置换植入物的整合和寿命这一目标做出了重要贡献。
