Shumiantseva V V, Bulko T V, Vagin M Iu, Suprun E V, Archakov A I
Biomed Khim. 2010 Nov-Dec;56(6):758-68. doi: 10.18097/pbmc20105606758.
Method targeting the direct monitoring of myoglobin based on analysis of electrochemical parameters of modified electrodes were proposed. Method of direct detection is based on interaction of myoglobin with anti-myoglobin with subsequent electrochemical registration of hemeprotein. Myocardial infarction biomarker myoglobin was quantified at biological level using screen printed electrodes modified with gold nanoparticles stabilized with didodecyldimethylammonium bromide (DDAB) and antibodies. Proposed method did not require signal enhancement and amplification and also labeled secondary antibodies. Electro analysis has high specificity and sensitivity. Myoglobin -antibodies interaction was studied also with electrochemical impedance spectroscopy. Sensor has low detection limit and broad diapason of working concentrations (17.8 ng/ml-1780 ng/ml; 1 nM-10 nM). Method based on gold nanoparticles detection on the surface of electrodes was treated for myoglobin identification. AuNP worked as an electrochemical sensing platform: the oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. The difference of cathodic peak area and peak high of gold oxide reduction in the case of electrodes with antibodies and electrodes with antibodies-myoglobin complex, was registered.
提出了基于修饰电极电化学参数分析直接监测肌红蛋白的方法。直接检测方法基于肌红蛋白与抗肌红蛋白的相互作用，随后对血红素蛋白进行电化学记录。使用用溴化二癸基二甲基铵（DDAB）稳定的金纳米颗粒和抗体修饰的丝网印刷电极，在生物水平上对心肌梗死生物标志物肌红蛋白进行了定量。所提出的方法不需要信号增强和放大，也不需要标记二抗。电化学分析具有高特异性和高灵敏度。还用电化学阻抗谱研究了肌红蛋白与抗体的相互作用。该传感器具有低检测限和宽工作浓度范围（17.8 ng/ml - 1780 ng/ml；1 nM - 10 nM）。基于电极表面金纳米颗粒检测的方法用于肌红蛋白识别。金纳米颗粒作为电化学传感平台：极化时金表面的氧化（导致形成金氧化物）作为分析响应的基础。记录了带有抗体的电极和带有抗体 - 肌红蛋白复合物的电极在金氧化物还原时阴极峰面积和峰高的差异。
