Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street 10, Moscow 119121, Russia.
Biosens Bioelectron. 2010 Mar 15;25(7):1694-8. doi: 10.1016/j.bios.2009.12.009. Epub 2009 Dec 16.
The myocardial infarction biomarker myoglobin was quantified at the biological level in undiluted plasma using developed electrochemical nanosensors with immobilized anti-myoglobin. Method for cardiac myoglobin detection is based on direct electron transfer between Fe(III)-heme and electrode surface modified with gold nanoparticles/didodecyldimethylammonium bromide (DDAB/Au) and antibodies. The procedure of myoglobin detection was optimized (pH, incubation times and characteristics of electrodes) to express determination of the marker in serum or plasma. Plasma of healthy donors and patients with acute myocardial infarction (AMI) was analyzed using electrochemical immunosensors and RAMP immunoassay. Square wave voltammetry cathodic peak of cardiac myoglobin reduction was found to be proportional to myoglobin quantity in plasma as determined by RAMP. The method proposed does not require signal enhancement or amplification; nor does it require labeled secondary antibodies. Immunosensor has a detection limit of 10 ng/ml (0.56 nM) and a broad range of working concentrations (10-1780 ng/ml; 0.56-100 nM). The whole procedure takes 30 min and can be used for express diagnosis of acute myocardial infarction.
心肌梗死生物标志物肌红蛋白在未稀释的血浆中使用固定化抗肌红蛋白的开发电化学纳米传感器在生物水平上进行定量。心肌肌红蛋白检测方法基于 Fe(III)-卟啉与电极表面的直接电子转移，该电极表面经过金纳米粒子/二癸基二甲基溴化铵（DDAB/Au）和抗体修饰。优化了肌红蛋白检测的步骤（pH、孵育时间和电极特性），以表达对血清或血浆中标志物的测定。使用电化学免疫传感器和 RAMP 免疫分析对健康供体和急性心肌梗死（AMI）患者的血浆进行了分析。发现心肌肌红蛋白还原的方波伏安阴极峰与 RAMP 测定的血浆中肌红蛋白的量成正比。所提出的方法不需要信号增强或放大；也不需要标记的二级抗体。免疫传感器的检测限为 10ng/ml（0.56nM），工作浓度范围较宽（10-1780ng/ml；0.56-100nM）。整个过程需要 30 分钟，可用于急性心肌梗死的快速诊断。
