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基于锰掺杂二氧化钛纳米粒子的无标记电化学传感器用于肌红蛋白检测：急性心肌梗死的生物标志物。

Label-Free Electrochemical Sensor Based on Manganese Doped Titanium Dioxide Nanoparticles for Myoglobin Detection: Biomarker for Acute Myocardial Infarction.

机构信息

Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha 62529, Saudi Arabia.

Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia (Central University), New Delhi 110025, India.

出版信息

Molecules. 2021 Jul 13;26(14):4252. doi: 10.3390/molecules26144252.

DOI:10.3390/molecules26144252
PMID:34299527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8306677/
Abstract

A label free electrochemical sensor based on pure titanium oxide and manganese (Mn)-doped titanium oxide (TiO) nanoparticles are fabricated and characterized for the sensitive detection of myoglobin (Mb) levels to analyze the cardiovascular infarction. Pristine and Mn-doped TiO nanoparticles were synthesized via the sol-gel method and characterized in order to understand their structure, morphologies, composition and optical properties. The structural properties revealed that the pure- and doped-TiO nanoparticles possess different TiO planes. FTIR studies confirm the formation of metal oxide nanoparticles by exhibiting a well-defined peak in the range of 600-650 cm. The values of the optical band gap, estimated from UV-Vis spectroscopy, are decreased for the Mn-doped TiO nanoparticles. UV-Vis spectra in the presence of myoglobin (Mb) indicated interaction between the TiO nanoparticles and myoglobin. The SPE electrodes were then fabricated by printing powder film over the working electrode and tested for label-free electrochemical detection of myoglobin (Mb) in the concentration range of 0-15 nM Mb. The fabricated electrochemical sensor exhibited a high sensitivity of 100.40 μA-cmnM with a lowest detection limit of 0.013 nM (0.22 ng/mL) and a response time of ≤10 ms for sample S3. An interference study with cyt-c and Human Serum Albumin (HSA) of the sensors show the selective response towards Mb in 1:1 mixture.

摘要

基于纯氧化钛和锰（Mn）掺杂氧化钛（TiO）纳米粒子的无标记电化学传感器被制备并进行了特性分析，用于灵敏检测肌红蛋白（Mb）水平，以分析心血管梗塞。通过溶胶-凝胶法合成了纯和 Mn 掺杂的 TiO 纳米粒子，并对其进行了结构、形貌、组成和光学性质的研究。结构性质表明，纯和掺杂的 TiO 纳米粒子具有不同的 TiO 平面。FTIR 研究通过在 600-650cm 范围内呈现出定义明确的峰来证实金属氧化物纳米粒子的形成。从紫外-可见光谱估算的光学带隙值降低了 Mn 掺杂的 TiO 纳米粒子。存在肌红蛋白（Mb）时的紫外-可见光谱表明 TiO 纳米粒子与肌红蛋白之间存在相互作用。然后通过在工作电极上打印粉末膜来制备 SPE 电极，并测试了无标记电化学检测 0-15 nM Mb 范围内肌红蛋白（Mb）的性能。所制备的电化学传感器表现出 100.40 μA-cmnM 的高灵敏度，最低检测限为 0.013 nM（0.22 ng/mL），对样品 S3 的响应时间≤10ms。传感器与 cyt-c 和人血清白蛋白（HSA）的干扰研究表明，其对 Mb 的选择性响应在 1:1 混合物中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/89eadc8a7384/molecules-26-04252-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/4ef1693107da/molecules-26-04252-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/9b26691a9853/molecules-26-04252-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/9eb17e645c01/molecules-26-04252-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/324c29f9cf45/molecules-26-04252-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/8c88fb0e311a/molecules-26-04252-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/47d9368ff8e6/molecules-26-04252-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/92fc5f5fe42a/molecules-26-04252-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/89eadc8a7384/molecules-26-04252-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/4ef1693107da/molecules-26-04252-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/9b26691a9853/molecules-26-04252-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/8b63cf8aea22/molecules-26-04252-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/9eb17e645c01/molecules-26-04252-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/324c29f9cf45/molecules-26-04252-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/8c88fb0e311a/molecules-26-04252-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/47d9368ff8e6/molecules-26-04252-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/92fc5f5fe42a/molecules-26-04252-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ca1/8306677/89eadc8a7384/molecules-26-04252-g009.jpg

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