各种商业检测试剂盒检测托斯卡纳病毒抗体的性能。
Performance of various commercial assays for the detection of Toscana virus antibodies.
机构信息
Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
出版信息
Vector Borne Zoonotic Dis. 2011 Jun;11(6):781-7. doi: 10.1089/vbz.2010.0224. Epub 2011 Mar 11.
INTRODUCTION
Sandfly fever virus (SFV) serotypes sandfly fever Naples virus, sandfly fever Sicilian virus, and sandfly fever Cyprus virus cause febrile diseases, whereas Toscana virus (TOSV) is responsible for aseptic meningoencephalitis. Diagnosis and surveillance of TOSV depend heavily on virus serology, and various commercial assays utilizing various antigen sources and formats have been available. The aim of this study was to perform comparative evaluation of commercially available serological assays for anti-TOSV immunoglobulins.
MATERIALS AND METHODS
A collection of 120 sera from healthy blood donors from an endemic region, previously identified to be reactive for antibodies against various SFV serotypes by indirect immunofluorescence test (IIFT), was reevaluated for IgG/IgM via IIFT, enzyme-linked immunosorbent assay, and an immunoblot assay manufactured by Euroimmun, Diesse, and Mikrogen, respectively. Virus neutralization test (VNT) was performed for 99 sera using standard TOSV, sandfly fever Sicilian virus, and sandfly fever Naples virus strains.
RESULTS
A total of 89 samples (74.2%) were reactive for TOSV IgG in at least one of the commercial assays, and 31 samples (31.3%) were reactive in VNT for various SFV serotypes. Average percentage agreements among commercial assays and between VNT and the commercial assays were noted as 57.8% and 62.6%, respectively. No significant correlation between assay results and VNT titers was observed. SFV IgM antibodies were detected in a total of eight samples (6.7%) via IIFT, which were nonreactive in enzyme-linked immunosorbent assay and VNT.
DISCUSSION
Commercial diagnostic immunoassays displayed slight to fair agreement for TOSV IgG as assessed via kappa and percentage agreement values. The results could only be confirmed via virus neutralization in a portion of the samples, and overall agreement between the commercial assays and VNT was slight. Commercial assays such as immunoblot can be used in addition to VNT for confirmation of TOSV exposure.
简介
沙蝇热病毒（SFV）血清型沙蝇热那不勒斯病毒、沙蝇热西西里病毒和沙蝇热塞浦路斯病毒引起发热性疾病，而托斯卡纳病毒（TOSV）则导致无菌性脑膜脑炎。TOSV 的诊断和监测严重依赖于病毒血清学，并且已经有各种利用不同抗原来源和形式的商业检测方法。本研究的目的是对商业上可获得的抗 TOSV 免疫球蛋白的血清学检测方法进行比较评估。
材料和方法
从一个流行地区的 120 份健康献血者血清中采集了一个集合，这些血清先前通过间接免疫荧光试验（IIFT）被鉴定为对各种 SFV 血清型的抗体呈反应性，然后通过 IIFT、酶联免疫吸附试验和免疫印迹试验分别由 Euroimmun、Diesse 和 Mikrogen 进行 IgG/IgM 再评估。使用标准 TOSV、沙蝇热西西里病毒和沙蝇热那不勒斯病毒株对 99 份血清进行病毒中和试验（VNT）。
结果
在至少一种商业检测方法中，共有 89 个样本（74.2%）对 TOSV IgG 呈反应性，31 个样本（31.3%）在 VNT 中对各种 SFV 血清型呈反应性。商业检测方法之间以及 VNT 和商业检测方法之间的平均百分比一致性分别为 57.8%和 62.6%。未观察到检测结果与 VNT 滴度之间存在显著相关性。通过 IIFT 共检测到 8 个样本（6.7%）的 SFV IgM 抗体，这些抗体在酶联免疫吸附试验和 VNT 中均无反应性。
讨论
通过 Kappa 和百分比一致性值评估，商业诊断免疫测定法对 TOSV IgG 的显示略有至良好的一致性。仅在部分样本中可通过病毒中和来确认结果，并且商业检测方法和 VNT 之间的总体一致性仅为轻度。免疫印迹等商业检测方法可与 VNT 一起用于确认 TOSV 暴露。
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