Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Eur J Neurosci. 2011 Mar;33(6):1025-36. doi: 10.1111/j.1460-9568.2011.07600.x.
Many laboratories have focused efforts on the creation of transgenic mouse models to study adult neurogenesis. In the last decade several constitutive reporter, as well as inducible transgenic lines have been published that allowed for visualization, tracking and alteration of specific neurogenic cell populations in the adult brain. Given the popularity of this approach, multiple mouse lines are available, and this review summarizes the differences in the basic techniques that have been used to create these mice, highlighting the different constructs and reporter proteins used, as well as the strengths and limitations of each of these models. Representative examples from the literature demonstrate some of the diverse and seminal findings that have come to fruition through the laborious, yet highly rewarding work of creating transgenic mouse lines for adult neurogenesis research.
许多实验室都致力于创建转基因小鼠模型来研究成年神经发生。在过去的十年中，已经发表了几个组成型报告基因以及诱导型转基因系，这些系允许在成年大脑中可视化、跟踪和改变特定的神经发生细胞群体。鉴于这种方法的普及，有多种小鼠系可供使用，本综述总结了用于创建这些小鼠的基本技术的差异，重点介绍了使用的不同构建体和报告蛋白，以及这些模型各自的优缺点。文献中的代表性例子展示了通过为成年神经发生研究创建转基因小鼠系的艰苦而又非常有价值的工作所取得的一些多样化的、重要的发现。
