Key Laboratory of Marine Genetics and Breeding for Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
J Appl Microbiol. 2011 Jun;110(6):1438-48. doi: 10.1111/j.1365-2672.2011.04998.x. Epub 2011 Apr 8.
The aim of this study was to detect the production of three kinds of quorum sensing (QS) signal molecules, i.e. the N-acyl-homoserine lactone (AHL), the autoinducer-2 (AI-2) and the cholerae autoinducer-1-like (CAI-1-like) molecules in 25 Vibrionaceae strains.
The QS signal molecules in 25 Vibrionaceae strains were detected with different biosensors. Except Salinivibrio costicola VIB288 and Vibrio natriegens VIB299, all the other 23 Vibrionaceae strains could produce one or more kinds of detectable QS signal molecules. Twenty-one of the 25 strains were found to produce AHL signal molecules by using Vibrio harveyi JMH612 and Agrobacterium tumefaciens KYC55 (pJZ372; pJZ384; pJZ410) as biosensors. The AHL fingerprints of eight strains were detected by thin-layer chromatography with Ag. tumefaciens KYC55, and two of them, i.e. V. mediterranei VIB296 and Aliivibrio logei VIB414 had a high diversity of AHLs. Twenty of the 25 strains were found to have the AI-2 activity, and the luxS gene sequences in 18 strains were proved to be conserved by PCR amplification and sequencing. Only six (five Vibrio strains and A. logei VIB414) of the 25 strains possessed the CAI-1-like activity. A. logei VIB414, V. campbellii VIB285, V. furnissii VIB293, V. pomeroyi LMG20537 and two V. harveyi strains VIB571 and VIB645 were found to produce all the three kinds of QS signal molecules.
The results indicated that the QS signal molecules, especially AHL and AI-2 molecules, were widespread in the family Vibrionaceae.
In response to a variety of environmental conditions and selection forces, the family Vibrionaceae produced QS signal molecules with great diversity and complexity. The knowledge we obtained from this study will be useful for further research on the roles of different QS signal molecules in this family.
本研究旨在检测 25 株弧菌科菌株产生的 3 种群体感应（QS）信号分子，即 N-酰基高丝氨酸内酯（AHL）、自诱导因子-2（AI-2）和霍乱弧菌诱导物-1 样（CAI-1 样）分子。
利用不同的生物传感器检测 25 株弧菌科菌株中的 QS 信号分子。除了盐单胞菌 VIB288 和海生奈氏球菌 VIB299 外，其他 23 株弧菌科菌株均可产生一种或多种可检测到的 QS 信号分子。利用哈维氏弧菌 JMH612 和根癌农杆菌 KYC55（pJZ372;pJZ384;pJZ410）作为生物传感器，发现 25 株菌中有 21 株可产生 AHL 信号分子。利用根癌农杆菌 KYC55 对 8 株菌的 AHL 指纹图谱进行薄层色谱检测，其中 2 株，即地中海弧菌 VIB296 和海洋发光杆菌 VIB414，AHL 种类丰富。发现 25 株菌中有 20 株具有 AI-2 活性，通过 PCR 扩增和测序证明 18 株菌的 luxS 基因序列保守。在 25 株菌中，只有 6 株（5 株弧菌和海洋发光杆菌 VIB414）具有 CAI-1 样活性。海洋发光杆菌 VIB414、坎贝尔氏弧菌 VIB285、芬尼斯弧菌 VIB293、波默罗伊氏弧菌 LMG20537 和 2 株哈维氏弧菌 VIB571 和 VIB645 被发现可产生所有 3 种 QS 信号分子。
结果表明，QS 信号分子，特别是 AHL 和 AI-2 分子，在弧菌科中广泛存在。
为了应对各种环境条件和选择压力，弧菌科产生了具有极大多样性和复杂性的 QS 信号分子。我们从这项研究中获得的知识将有助于进一步研究该家族中不同 QS 信号分子的作用。
