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弧菌科 57 个成员中酰基高丝氨酸内酯的存在。

Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family.

机构信息

The Norwegian Structural Biology Centre, University of Tromsø, Tromsø, Norway.

出版信息

J Appl Microbiol. 2013 Sep;115(3):835-47. doi: 10.1111/jam.12264. Epub 2013 Jun 28.

DOI:10.1111/jam.12264
PMID:23725044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3910146/
Abstract

AIMS

The aim of this study was to use a sensitive method to screen and quantify 57 Vibrionaceae strains for the production of acyl-homoserine lactones (AHLs) and map the resulting AHL profiles onto a host phylogeny.

METHODS AND RESULTS

We used a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) protocol to measure AHLs in spent media after bacterial growth. First, the presence/absence of AHLs (qualitative analysis) was measured to choose internal standard for subsequent quantitative AHL measurements. We screened 57 strains from three genera (Aliivibrio, Photobacterium and Vibrio) of the same family (i.e. Vibrionaceae). Our results show that about half of the isolates produced multiple AHLs, typically at 25-5000 nmol l(-1) .

CONCLUSIONS

This work shows that production of AHL quorum sensing signals is found widespread among Vibrionaceae bacteria and that closely related strains typically produce similar AHL profiles.

SIGNIFICANCE AND IMPACT OF THE STUDY

The AHL detection protocol presented in this study can be applied to a broad range of bacterial samples and may contribute to a wider mapping of AHL production in bacteria, for example, in clinically relevant strains.

摘要

目的

本研究旨在使用灵敏的方法筛选和定量分析 57 株弧菌科菌株产生酰高丝氨酸内酯 (AHLs)的情况，并将所得 AHL 图谱映射到宿主系统发育上。

方法和结果

我们使用高效液相色谱-串联质谱 (HPLC-MS/MS) 方案测量细菌生长后培养液中 AHLs 的含量。首先，通过测量 AHLs 的存在/不存在（定性分析）来选择后续定量 AHL 测量的内标。我们从同一科（弧菌科）的三个属（Aliivibrio、Photobacterium 和 Vibrio）中筛选了 57 株菌株。我们的结果表明，大约一半的分离株产生多种 AHLs，通常在 25-5000 nmol l(-1)之间。

结论

这项工作表明，AHL 群体感应信号的产生在弧菌科细菌中广泛存在，并且密切相关的菌株通常产生相似的 AHL 图谱。

研究的意义和影响

本研究中提出的 AHL 检测方案可应用于广泛的细菌样本，并可能有助于更广泛地绘制细菌中 AHL 产生的图谱，例如在临床上相关的菌株中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6772/3910146/157e6e460638/jam0115-0835-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6772/3910146/43e7c97b7439/jam0115-0835-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6772/3910146/157e6e460638/jam0115-0835-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6772/3910146/43e7c97b7439/jam0115-0835-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6772/3910146/157e6e460638/jam0115-0835-f2.jpg

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