Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, South Korea.
Biochem Biophys Res Commun. 2011 Apr 1;407(1):266-70. doi: 10.1016/j.bbrc.2011.03.023. Epub 2011 Mar 23.
It has been shown that porcine endogenous retrovirus (PERV) can infect human cells, indicating that PERV transmission poses a serious concern in pig-to-human xenotransplantation. A number of recent studies have reported on retrovirus interference by antiviral proteins. The most potent antiviral proteins are members of the APOBEC family of cytidine deaminases, which are involved in defense against retroviral attack. These proteins are present in the cytoplasm of mammalian cells and inhibit retroviral replication. To evaluate the inhibition of PERV transmission by human APOBEC3 proteins, we co-transfected 293T cells with a PERV molecular clone and human APOBEC3F or APOBEC3G expression vectors, and monitored PERV replication competency using a quantitative analysis of PERV pol genes. The replication of PERVs in cells co-expressing human APOBEC3s was reduced by 60-90% compared with PERV-only control. These results suggest that human APOBEC3G and APOBEC3F might serve a potential barrier function against PERV transmission in xenotransplantation.
已经表明，猪内源性逆转录病毒（PERV）可以感染人类细胞，这表明 PERV 的传播在猪到人异种移植中是一个严重的问题。最近的一些研究报告了抗病毒蛋白对逆转录病毒的干扰。最有效的抗病毒蛋白是 APOBEC 家族的胞嘧啶脱氨酶成员，它们参与了抵御逆转录病毒攻击的过程。这些蛋白质存在于哺乳动物细胞的细胞质中，并抑制逆转录病毒的复制。为了评估人 APOBEC3 蛋白对 PERV 传播的抑制作用，我们将 PERV 分子克隆和人 APOBEC3F 或 APOBEC3G 表达载体共转染 293T 细胞，并使用 PERV pol 基因的定量分析来监测 PERV 复制能力。与仅 PERV 对照相比，共表达人 APOBEC3 的细胞中 PERV 的复制减少了 60-90%。这些结果表明，人 APOBEC3G 和 APOBEC3F 可能在异种移植中发挥潜在的屏障功能，防止 PERV 的传播。
