Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
Eur J Pharm Biopharm. 2011 Sep;79(1):108-18. doi: 10.1016/j.ejpb.2011.03.002. Epub 2011 Mar 9.
The structure and functions of polymer nanofibers as wound dressing materials have been well investigated over the last few years. However, during the healing process, nanofibrous mats are inevitably involved in dynamic interactions with the wound environment, an aspect not explored yet. Potential active participation of ultrafine fibers as wound dressing material in a dynamic interaction with wound bacteria has been examined using three wound bacterial strains and antimicrobial fusidic acid (FA)-loaded electrospun PLGA ultrafine fibers (UFs). These were developed and characterized for morphology and in-use pharmaceutical attributes. In vitro microbiological studies showed fast bacterial colonization of UFs and formation of a dense biofilm. Interestingly, bacterial stacks on UFs resulted in a remarkable enhancement of drug release, which was associated with detrimental changes in morphology of UFs in addition to a decrease in pH of their aqueous incubation medium. In turn, UFs by allowing progressively faster release of bioactive FA eradicated planktonic bacteria and considerably suppressed biofilm. Findings point out the risk of wound reinfection and microbial resistance upon using non-medicated or inadequately medicated bioresorbable fibrous wound dressings. Equally important, data strongly draw attention to the importance of characterizing drug delivery systems and establishing material-function relationships for biomedical applications under biorelevant conditions.
在过去的几年中，人们对聚合物纳米纤维作为伤口敷料材料的结构和功能进行了广泛的研究。然而，在愈合过程中，纳米纤维垫不可避免地会与伤口环境发生动态相互作用，这一方面尚未得到探索。本研究使用三种伤口细菌菌株和载有抗菌药物夫西地酸（FA）的静电纺丝聚乳酸-羟基乙酸共聚物（PLGA）纳米纤维（UFs），研究了作为伤口敷料材料的超细纤维在与伤口细菌的动态相互作用中可能的主动参与。开发并表征了这些纤维的形态和使用中的药物属性。体外微生物学研究表明，UFs 上的细菌迅速定植并形成致密的生物膜。有趣的是，UFs 上的细菌堆积导致药物释放显著增加，这与 UF 的形态发生有害变化以及其水孵育介质的 pH 值下降有关。反过来，UFs 通过允许越来越快地释放生物活性 FA，根除了浮游细菌，并大大抑制了生物膜。研究结果指出，在使用非药物或药物不足的生物可吸收纤维状伤口敷料时，存在伤口再次感染和微生物耐药的风险。同样重要的是，数据强烈提醒人们注意在生物相关条件下对药物输送系统进行表征并建立材料功能关系的重要性，这对于生物医学应用至关重要。
