Department of Reproduction and Artificial Insemination, Istanbul University, Avcilar, Istanbul, Turkey.
Theriogenology. 2011 Jun;75(9):1647-51. doi: 10.1016/j.theriogenology.2011.01.004. Epub 2011 Mar 11.
There have not been successful and repeatable methods for in vitro embryo production in the dog. Up to date, only one blactocyst has been achieved on in vitro culture. Since reproductive physiology of the dog is different from that of other mammalian species, it seems that a suitable method for in vitro production of canine embryos is still far from being designed and routinely applied, and an effective protocol is needed. Therefore, the aim of the present study was to examine the effects of adding hormones sequentially, for mimicking the dog's in vivo endocrine milieu, on maturation of immature dog oocytes in vitro. At the end of the 96 h IVM period, nuclear maturation rates were evaluated by the aceto-orcein staining method. In comparison relating IVM rates, the sequential hormone addition was more beneficial on IVM rates (MI + MII) than the traditional hormone addition and control groups (48.1%, 38.9% and 23.0% respectively; P < 0.0001). As a result, hormone addition sequentially may be an effective approach for the IVM of the immature dog oocytes. We suggest that attempts to define the adequate conditions for IVM in the dog should extend towards this new perspective.
目前，犬的体外胚胎生产还没有成功且可重复的方法。迄今为止，仅在体外培养中获得了一个囊胚。由于犬的生殖生理学与其他哺乳动物物种不同，因此似乎仍然远未设计出适合犬胚胎体外生产的方法，并且需要有效的方案。因此，本研究的目的是研究顺序添加激素以模拟犬体内内分泌环境对体外成熟犬卵母细胞的影响。在 96 小时 IVM 期结束时，通过醋酸-orcein 染色法评估核成熟率。与 IVM 率的比较，与传统激素添加和对照组相比，顺序激素添加对 IVM 率（MI+MII）更有利（分别为 48.1％，38.9％和 23.0％；P <0.0001）。因此，顺序添加激素可能是体外成熟犬卵母细胞的有效方法。我们建议，为犬的 IVM 定义适当条件的尝试应朝着这一新视角扩展。
