Laboratory of Applied Microbiology, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
J Biosci Bioeng. 2011 Jun;111(6):671-4. doi: 10.1016/j.jbiosc.2011.02.001. Epub 2011 Mar 10.
An actinomycete that excretes inulin fructotransferase to the culture supernatant was able to produce di-d-fructofuranose 1,2':2,3' dianhydride (DFA III) from inulin, with the greatest rate of enzyme activity at 65°C and at a pH of 5.5. Through chemotaxonomic and 16S rRNA gene analysis, this strain was identified as genus Nonomuraea in the Streptosporangiaceae family. This is the first report of an inulin fructotransferase producer in this family.
一株能向培养液上清中分泌菊粉果糖转移酶的放线菌，能够将菊粉转化为二-d-果糖 1,2':2,3' 二酐（DFA III），该酶在 65°C 和 pH5.5 时具有最大的酶活。通过化学分类和 16S rRNA 基因分析，该菌株被鉴定为链霉菌科中的野野村菌属。这是该科中首例报道的菊粉果糖转移酶产生菌。
