Rahmani Nanik, Kashiwagi Norimasa, Lee JaeMin, Niimi-Nakamura Satoko, Matsumoto Hana, Kahar Prihardi, Lisdiyanti Puspita, Prasetya Bambang, Ogino Chiaki, Kondo Akihiko
Research Center for Biotechnology, Indonesian Institute of Sciences, Komplek CSC-LIPI, Jl. Raya Bogor Km.46, Cibinong, 16911, West Java, Indonesia.
Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Hyogo, Japan.
AMB Express. 2017 Dec;7(1):100. doi: 10.1186/s13568-017-0401-6. Epub 2017 May 19.
Mannan endo-1,4-β-mannosidase (commonly known as β-mannanase) catalyzes a random cleavage of the β-D-1,4-mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocellulose biomass, as well as in production of mannooligosaccharides (MOS) for applications in feed and food industries. We aimed to obtain a β-mannanase, for such mannan polymer utilization, from actinomycetes strains isolated in Indonesia. Strains exhibiting high mannanase activity were screened, and one strain belonging to the genus Kitasatospora was selected. We obtained a β-mannanase from this strain, and an amino acid sequence of this Kitasatospora β-mannanase showed a 58-71% similarity with the amino acid sequences of Streptomyces β-mannanases. The Kitasatospora β-mannanase showed a significant level of activity (944 U/mg) against locust bean gum (0.5% w/v) and a potential for oligosaccharide production from various mannan polymers. The β-mannanase might be beneficial particularly in the enzymatic production of MOS for applications of mannan utilization.
甘露聚糖内切 -1,4-β-甘露糖苷酶（通常称为β-甘露聚糖酶）催化甘露聚糖聚合物中β-D-1,4-甘露吡喃糖基键的随机断裂。该酶已被用于从木质纤维素生物质生产生物燃料，以及用于饲料和食品工业的低聚甘露糖（MOS）的生产。我们旨在从印度尼西亚分离的放线菌菌株中获得一种用于此类甘露聚糖聚合物利用的β-甘露聚糖酶。筛选出表现出高甘露聚糖酶活性的菌株，并选择了一株属于北里孢菌属的菌株。我们从该菌株中获得了一种β-甘露聚糖酶，该北里孢菌β-甘露聚糖酶的氨基酸序列与链霉菌β-甘露聚糖酶的氨基酸序列显示出58 - 71%的相似性。北里孢菌β-甘露聚糖酶对刺槐豆胶（0.5% w/v）表现出显著水平的活性（944 U/mg），并且具有从各种甘露聚糖聚合物生产低聚糖的潜力。该β-甘露聚糖酶可能特别有利于甘露聚糖利用应用中MOS的酶促生产。
