College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Chemosphere. 2011 Jun;84(1):124-30. doi: 10.1016/j.chemosphere.2011.02.034. Epub 2011 Mar 11.
Cypermethrin (CYP) is one of the most common contaminants in the ecosystem. The effects of CYP exposure on the induction of oxidative stress and endocrine disruption were studied in adolescent male ICR mice. The hepatic activities of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and total antioxidant capacity (T-AOC) increased significantly after 3 weeks (postnatal day 21-42) of oral administration of 20 mg kg(-1) CYP. In accordance with the enzyme activities, the mRNA levels for the genes encoding these antioxidant proteins, such as Sod1, Sod2, Gpx1 and Gpx2, were also up-regulated significantly in the 10 and 20 mg kg(-1) CYP treatment groups. Furthermore, we also found that the 3-week oral administration of CYP decreased transcription levels of key genes in pathways of cholesterol synthesis and transport and testosterone synthesis including HMG-CoA synthase, steroidogenic acute regulatory protein (StAR) and cytochrome P450 17α-hydroxysteroid dehydrogenase (P450 17α in the liver and testes. Serum testosterone levels also decreased significantly in mice after treatment with 20 mg kg(-1) CYP. Taken together, the results indicated that CYP can induce endocrine disruption in adolescent mice. The findings will be helpful in elucidating the mechanism of toxicity induced by CYP in adolescent mice.
氯菊酯（CYP）是生态系统中最常见的污染物之一。本研究旨在探讨 CYP 暴露对青春期雄性 ICR 小鼠氧化应激和内分泌干扰的影响。经口给予 20mg/kg CYP 3 周（生后第 21-42 天）后，肝脏中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、过氧化氢酶（CAT）和总抗氧化能力（T-AOC）等抗氧化酶的活性显著升高。与酶活性变化一致，编码这些抗氧化蛋白的基因，如 Sod1、Sod2、Gpx1 和 Gpx2 的 mRNA 水平在 10 和 20mg/kg CYP 处理组中也显著上调。此外，我们还发现，3 周 CYP 经口给药降低了肝脏和睾丸中胆固醇合成和转运以及睾酮合成途径中关键基因的转录水平，包括 HMG-CoA 合酶、类固醇急性调节蛋白（StAR）和细胞色素 P450 17α-羟化酶/脱氢酶（P450 17α）。经 20mg/kg CYP 处理后，小鼠血清睾酮水平也显著降低。综上所述，这些结果表明 CYP 可诱导青春期小鼠发生内分泌干扰。该研究结果有助于阐明 CYP 对青春期小鼠毒性作用的机制。
