Jin Yuanxiang, Wang Linggang, Chen Guanliang, Lin Xiaojian, Miao Wenyu, Fu Zhengwei
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Environ Toxicol Pharmacol. 2014 Mar;37(2):782-90. doi: 10.1016/j.etap.2014.02.014. Epub 2014 Feb 26.
Effects of atrazine (ATZ) and its metabolite diaminochlorotriazine (DACT) on the induction of oxidative stress and endocrine disruption were studied in mice. Body and liver weights decreased in all ATZ and DACT treated groups. Hepatic activities of superoxide dismutase (SOD) increased significantly after 1 week of intraperitoneal injection of 200 mg/kg ATZ, 100 and 200 mg/kg DACT. Hepatic activities of catalase (CAT) and glutathione S-transferase (GST) were also affected by the treatment with 200 mg/kg DACT. In serum, the glutathione peroxidase (GPX) and GST activities and glutathione (GSH) content decreased significantly in the 200 mg/kg DACT treated group. Moreover, the administration of ATZ and DACT decreased the transcription levels of key genes related to cholesterol transport and testosterone (T) synthesis including scavenger receptor class B type 1 (SR-B1), cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc) and cytochrome P450 17α-hydroxysteroid dehydrogenase (P450 17α) in testes. Furthermore, the treatment with 200 mg/kg DACT significantly decreased the serum and testicular T levels, while the treatment with 200 mg/kg ATZ significantly decreased the testicular T levels. The results indicated that the acute exposure to ATZ and DACT induced oxidative stress and endocrine disruption in mice, and DACT showed much more toxic than ATZ did.
研究了阿特拉津（ATZ）及其代谢物二氨基氯三嗪（DACT）对小鼠氧化应激诱导和内分泌干扰的影响。所有ATZ和DACT处理组的体重和肝脏重量均下降。腹腔注射200 mg/kg ATZ、100和200 mg/kg DACT 1周后，肝脏超氧化物歧化酶（SOD）活性显著增加。过氧化氢酶（CAT）和谷胱甘肽S-转移酶（GST）的肝脏活性也受到200 mg/kg DACT处理的影响。在血清中，200 mg/kg DACT处理组的谷胱甘肽过氧化物酶（GPX）和GST活性以及谷胱甘肽（GSH）含量显著降低。此外，ATZ和DACT的给药降低了睾丸中与胆固醇转运和睾酮（T）合成相关的关键基因的转录水平，包括B类清道夫受体1型（SR-B1）、细胞色素P450胆固醇侧链裂解酶（P450scc）和细胞色素P450 17α-羟基类固醇脱氢酶（P450 17α）。此外，200 mg/kg DACT处理显著降低了血清和睾丸中的T水平，而200 mg/kg ATZ处理显著降低了睾丸中的T水平。结果表明，急性暴露于ATZ和DACT会诱导小鼠氧化应激和内分泌干扰，且DACT的毒性比ATZ大得多。
