Department of Pharmaceutical Engineering, School of Chemical Engineering, Wuhan University of Technology, Wuhan, PR China.
Biomaterials. 2011 Jul;32(20):4506-16. doi: 10.1016/j.biomaterials.2011.02.023. Epub 2011 Mar 11.
Biodegradable PDLLA/Chondroitin sulfate/Chitosan(PDLLA/CS/CHS) nerve conduits with potentially good biocompatibility and good mechanical property feasible for surgical manipulation have been developed in our previous work. The purpose of this study was to investigate their possible application in repairing damaged nerves and the effect of nerve growth factor (NGF). The PDLLA/CS/CHS/NGF nerve conduits were prepared by immobilizing NGF onto the PDLLA/CS/CHS nerve conduits with carbodiimide. Adult Sprague-Dawley (SD) rats weighing 200-250 g were used as the animal model. The conduits were employed to bridge the 10 mm defects in the sciatic nerve of the SD rats. Nerve conduction velocities (NCVs) were clearly detected in both nerve conduits after 3 months of implantation, indicating a rapid functional recovery for the disrupted nerves. The results of histological sections showed that the internal sides of the conduits were compact enough to prevent the connective tissues from ingrowth. Combined with the strong mechanical properties, good nerve regeneration ability and non-toxicity of its degradation products, PDLLA/CS/CHS nerve conduits would be expected to be useful materials to repair nerve damage and NGF can effectively promote the regeneration of peripheral nerve defect.
在我们之前的工作中，已经开发出了具有潜在良好生物相容性和良好机械性能的可生物降解 PDLLA/硫酸软骨素/壳聚糖（PDLLA/CS/CHS）神经导管，便于手术操作。本研究旨在探讨其在修复受损神经和神经生长因子（NGF）方面的可能应用。通过将 NGF 固定在 PDLLA/CS/CHS 神经导管上来制备 PDLLA/CS/CHS/NGF 神经导管。使用体重为 200-250 g 的成年 Sprague-Dawley（SD）大鼠作为动物模型。将导管用于桥接 SD 大鼠坐骨神经的 10 mm 缺损。植入 3 个月后，在两个神经导管中均清楚地检测到神经传导速度（NCVs），表明受损神经的功能迅速恢复。组织学切片的结果表明，导管的内部足够紧密，可防止结缔组织向内生长。结合其良好的机械性能、神经再生能力和降解产物的非毒性，PDLLA/CS/CHS 神经导管有望成为修复神经损伤的有用材料，并且 NGF 可以有效促进周围神经缺损的再生。
