Acústica Amplivox, Pelotas, Brazil.
Pathol Res Pract. 2011 Apr 15;207(4):232-5. doi: 10.1016/j.prp.2011.02.002. Epub 2011 Mar 11.
This study presents the development and evaluation of an image processing software for computer-assisted cellular structure counting. The proposed software consists of a set of processing and analytical tools which allows its use in several applications of cell and cellular structure counting. A particular application in AgNOR quantitative analysis is presented. The knowledge obtained from experienced pathologists has been codified in a sequence of processing steps in order to allow automatic estimation of the mean number of AgNORs per cell in ameloblastomas. The performance of the presented software in such application was verified by comparing the data provided by visual analysis, by two observers previously calibrated and under supervision of two experienced specialists (Group 1) and by the computer program (Group 2). No statistical difference was observed (p<0.05) between the two groups. The use of the proposed method in AgNOR applications permitted attainment of accurate and precise data without the difficulties frequently found in the traditional visual analysis method (time, training and subjectivity). The developed software is an interesting tool as an aid in the study (estimation of the number of cells and cellular structures) of malignant and benign neoplasms.
本研究提出了一种用于计算机辅助细胞结构计数的图像处理软件的开发和评估。所提出的软件由一组处理和分析工具组成，允许在细胞和细胞结构计数的几个应用中使用。本文提出了一个在 AgNOR 定量分析中的应用。已经将经验丰富的病理学家的知识编码为一系列处理步骤，以便能够自动估计成釉细胞瘤中每个细胞的 AgNOR 平均数量。通过将由两个经过校准的观察者在两个经验丰富的专家的监督下（第 1 组）进行的视觉分析所提供的数据与计算机程序（第 2 组）进行比较，验证了所提出的软件在这种应用中的性能。两组之间没有观察到统计学差异（p<0.05）。该方法在 AgNOR 应用中的使用允许获得准确和精确的数据，而没有传统视觉分析方法中经常遇到的困难（时间、培训和主观性）。所开发的软件是一种有趣的工具，可作为研究（估计恶性和良性肿瘤的细胞和细胞结构数量）的辅助工具。
