Müntener Tabitha, Doherr Marcus G, Guscetti Franco, Suter Maja M, Welle Monika M
Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, CH-3012 Bern, Switzerland.
Vet Dermatol. 2011 Oct;22(5):383-95. doi: 10.1111/j.1365-3164.2011.00963.x. Epub 2011 Mar 14.
The hair follicle has a lifelong capacity to cycle through recurrent phases of controlled growth (anagen), regression (catagen) and quiescence (telogen), each associated with specific morphological changes. A comprehensive classification scheme is available for mice to distinguish the cycle stages anagen I-VI, catagen I-VIII and telogen. For dogs, such a classification system does not exist, although alopecia associated with hair cycle arrest is common. We applied analogous morphological criteria and various staining techniques to subdivide the canine hair cycle stages to the same extent as has been done in mice. Of all the staining techniques applied, haematoxylin and eosin stain, Sacpic, Masson Fontana and immunohistochemistry for vimentin and laminin proved to be most useful. To evaluate the applicability of our criteria, we investigated skin biopsies from healthy beagle dogs (n=20; biopsies from shoulder and thigh) kept in controlled conditions. From each biopsy, at least 50 hair follicles were assessed. Statistical analysis revealed that 30% of the follicles were in anagen (12% early and 18% late), 8% in catagen (2% early, 5% late and 1% not determinable) and 27% in telogen. Thirty-five per cent of hair follicles could not be assigned to a specific cycle stage because not all follicles within one biopsy were oriented perfectly. In conclusion, this guide will not only be helpful for the investigation of alopecic disorders and possibly their pathogenesis, but may also serve as a basis for research projects in which the comparison of hair cycle stages is essential, e.g. comparative analysis of gene expression patterns.
毛囊具有终生循环经历可控生长（生长期）、退化（退行期）和静止（休止期）反复阶段的能力，每个阶段都伴有特定的形态学变化。小鼠有一个全面的分类方案来区分生长期I-VI、退行期I-VIII和休止期的循环阶段。对于犬类，虽然与毛发周期停滞相关的脱发很常见，但这样的分类系统并不存在。我们应用类似的形态学标准和各种染色技术，将犬类毛发周期阶段细分到与小鼠相同的程度。在所有应用的染色技术中，苏木精和伊红染色、Sacpic染色、Masson Fontana染色以及波形蛋白和层粘连蛋白的免疫组织化学染色被证明是最有用的。为了评估我们标准的适用性，我们调查了在受控条件下饲养的健康比格犬（n=20；取自肩部和大腿的活检样本）的皮肤活检样本。从每个活检样本中评估至少50个毛囊。统计分析显示，30%的毛囊处于生长期（12%为早期，18%为晚期），8%处于退行期（2%为早期，5%为晚期，1%无法确定），27%处于休止期。35%的毛囊无法归为特定的周期阶段，因为一个活检样本中的所有毛囊并非都完美定向。总之，本指南不仅有助于脱发疾病及其可能的发病机制的研究，还可能作为毛发周期阶段比较至关重要的研究项目的基础，例如基因表达模式的比较分析。
