Wolfe Ralph S
Department of Microbiology, University of Illinois, Urbana, Illinois, USA.
Methods Enzymol. 2011;494:1-22. doi: 10.1016/B978-0-12-385112-3.00001-9.
Basic techniques for the cultivation of methanogenic archaea in anoxic media, where the O/R potential is maintained below (-) 330 mV under a pressurized atmosphere of 20% carbon dioxide, are described.
描述了在缺氧培养基中培养产甲烷古菌的基本技术,其中在20%二氧化碳的加压气氛下,氧化还原电位保持在(-)330 mV以下。