Nishino Yudai, Ebisawa Katsumi, Yamada Yoichi, Okabe Kazuto, Kamei Yuzuru, Ueda Minoru
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
J Craniofac Surg. 2011 Mar;22(2):438-42. doi: 10.1097/SCS.0b013e318207b507.
In this research, we examined the effect on wound healing applying basic fibroblast growth factor (b-FGF) that is approved for clinical use to enhance wound healing and human deciduous teeth dental pulp cells (hDPCs) in clinics, but that have been attracting attention as a novel stem cell source in recent years. Human deciduous teeth were harvested from healthy volunteers, and hDPCs were isolated. We used a nude mouse full-thickness skin defect model and evaluated wound healing by macroscopic view and histologic and histomorphometric analysis. The mice were randomly divided into 4 groups: phosphate-buffered saline-treated group (control group), b-FGF-treated group (b-FGF group), hDPC-treated group (hDPC group), and hDPC and b-FGF-treated group (hDPC/b-FGF group). Basic fibroblast growth factor and hDPC groups accelerated wound healing compared with the control group. There was no statistically significant difference in wound healing observed between the hDPC and b-FGF groups. The hDPC/b-FGF group demonstrated accelerated wound healing compared with other groups. At day 14, PKH26-positive cells were surrounded by human type I collagen in hDPC and hDPC/b-FGF groups in immunohistologic evaluation. Significantly increased collagen fibril areas in wound tissues were observed in b-FGF, hDPC, and hDPC/b-FGF groups as compared with the control group at days 7 and 14. Our results showed that the hDPC/b-FGF group significantly promotes wound healing compared with other groups. This study implies that deciduous teeth that are currently considered as medical spare parts might offer a unique stem cell resource for potential of new cell therapies for wound healing in combination with b-FGF.
在本研究中，我们检测了临床批准用于促进伤口愈合的碱性成纤维细胞生长因子（b-FGF）和人乳牙牙髓细胞（hDPCs）对伤口愈合的影响，近年来，hDPCs作为一种新型干细胞来源受到关注。从健康志愿者处采集人乳牙，并分离出hDPCs。我们使用裸鼠全层皮肤缺损模型，通过宏观观察、组织学和组织形态计量学分析评估伤口愈合情况。将小鼠随机分为4组：磷酸盐缓冲盐水处理组（对照组）、b-FGF处理组（b-FGF组）、hDPC处理组（hDPC组）和hDPC与b-FGF联合处理组（hDPC/b-FGF组）。与对照组相比，碱性成纤维细胞生长因子组和hDPC组加速了伤口愈合。hDPC组和b-FGF组在伤口愈合方面未观察到统计学上的显著差异。与其他组相比，hDPC/b-FGF组伤口愈合加速。在第14天，免疫组织学评估显示，hDPC组和hDPC/b-FGF组中PKH26阳性细胞被人I型胶原包围。与对照组相比，在第7天和第14天，b-FGF组、hDPC组和hDPC/b-FGF组伤口组织中的胶原纤维面积显著增加。我们的结果表明，与其他组相比，hDPC/b-FGF组显著促进伤口愈合。本研究表明，目前被视为医疗备用部件的乳牙可能为结合b-FGF进行伤口愈合的新细胞疗法提供独特的干细胞资源。
