Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.
Opt Lett. 2011 Mar 15;36(6):912-4. doi: 10.1364/OL.36.000912.
We present a study of the three-dimensional structure of cancer cells using dual-wavelength phase-imaging digital holographic microscopy. Phase imaging of objects with optical height variation greater than the wavelength of light is ambiguous and causes phase wrapping. By comparing two phase images recorded at different wavelengths, the images can be accurately unwrapped. The unwrapping method is computationally fast and straightforward, and it can process complex topologies. Additionally, the limitations on the total optical height are significantly relaxed. This new methodology is widely applicable to other phase-imaging techniques as well as in applications beyond optical microscopy.
我们使用双波长相衬数字全息显微镜研究了癌细胞的三维结构。对于光学高度变化大于光波长的物体进行相衬成像存在歧义，会导致相位包裹。通过比较在两个不同波长下记录的两幅相位图像，可以准确地解包裹。该解包裹方法计算速度快、简单直接，可以处理复杂的拓扑结构。此外，对总光学高度的限制也得到了显著放宽。这种新方法广泛适用于其他相衬成像技术以及光学显微镜以外的应用。
