Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540 Naruda, Odawara, Kanagawa, 250-0862, Japan.
Cytotechnology. 2011 Mar;63(2):133-41. doi: 10.1007/s10616-010-9321-x. Epub 2011 Mar 15.
Involvement of impaired peritoneal immunosurveillance systems has been well established in the pathology of endometriosis. On the other hand, it has been observed that peritoneal administration of IL-12 suppress development of endometriotic lesions in a mouse endometriosis model. We investigated the effect of peritoneal administration of IL-12 on the peritoneal immunosurveillance system regarding NK cells in the mouse model. Treating the endometrial-tissue challenged mice with IL-12 for 5 consecutive days, from day -2 to day 2 (implantation of the endometrial tissues was done on day 0), cytotoxicity of splenic NK cells was enhanced immediately after the administration, on day 3, and development of the endometriotic lesions was reduced on day 21. In vivo NK cell depletion by administration of anti-IL-2Rβ mAb resulted in reduction of the cytotoxicity of splenic NK cells concomitant with a significant attenuation of suppressive effect of IL-12 on development of endometriotic lesions. Therefore, it was suggested that IL-12 suppresses development of endometriotic lesions via activation of NK cells, and that NK cells are involved in the primary defense for the development of endometriotic lesions.
在子宫内膜异位症的病理学中，已经充分证实了受损的腹膜免疫监视系统的参与。另一方面，人们观察到，在小鼠子宫内膜异位症模型中，腹腔内给予白细胞介素-12（IL-12）可抑制子宫内膜异位病灶的发展。我们研究了腹腔内给予 IL-12 对 NK 细胞的腹膜免疫监视系统在小鼠模型中的影响。用 IL-12 连续 5 天处理子宫内膜组织 challenged 的小鼠，从第-2 天到第 2 天（第 0 天进行子宫内膜组织植入），给药后立即增强脾 NK 细胞的细胞毒性，第 3 天，第 21 天减少子宫内膜异位病灶的发展。通过给予抗 IL-2Rβ mAb 进行体内 NK 细胞耗竭导致脾 NK 细胞的细胞毒性降低，同时显著减弱了 IL-12 对子宫内膜异位病灶发展的抑制作用。因此，据推测，IL-12 通过激活 NK 细胞抑制子宫内膜异位病灶的发展，而 NK 细胞参与子宫内膜异位病灶发展的初级防御。
