Witte A, Bläsi U, Halfmann G, Szostak M, Wanner G, Lubitz W
Institute of Microbiology and Genetics, University of Vienna, Austria.
Biochimie. 1990 Feb-Mar;72(2-3):191-200. doi: 10.1016/0300-9084(90)90145-7.
Bacteriophage PhiX174 encodes a single lysis gene, E, the function of which is necessary and sufficient to induce lysis of Escherichia coli. Here we present a novel model for E-lysis: physiological, genetic and biochemical data are presented which suggest that a transmembrane tunnel penetrating the inner and outer membrane is formed during the lytic action of protein E. Moreover, using high magnification scanning and transmission electron microscopy in this study, it was possible to visualize the transmembrane lysis structure directly.
噬菌体PhiX174编码一个单一的裂解基因E，其功能对于诱导大肠杆菌裂解而言是必要且充分的。在此，我们提出了一种关于E介导裂解的新模型：我们展示了生理学、遗传学和生物化学数据，这些数据表明在蛋白质E的裂解作用过程中形成了一个贯穿内膜和外膜的跨膜通道。此外，在本研究中使用高倍扫描和透射电子显微镜，可以直接观察到跨膜裂解结构。
