Nihon Medi-Physics Co., Ltd., N-7, W-1-2-6, Kita-ka, 060-0807, Sapporo, Hokkaido, Japan.
Environ Health Prev Med. 2001 Apr;6(1):22-6. doi: 10.1007/BF02897305.
We performed this study to evaluate the chronic effect of training on neutrophil functions in humans. Twenty-six university students (14 males and 12 females) with a mean age of 19.1±0.8 years were divided into the athlete group and the control group based on answers given to a written questionnaire. The capacity of circulating neutrophils to ingest bacteria (phagocytosis) and to produce superoxide (nitroblue tetrazolium reduction) was measured under resting conditions in the absence of training activities. In addition, we measured hematological and serum biochemical parameters.The present analyses revealed that the frequency of subjective symptoms concerning susceptibility to infections (sum of males and females) was higher in the athlete group than in the control group (p<0.05). In the investigation of males, hemoglobin and serum protein levels of the athlete group were lower than that of the control group (p<0.01, p<0.05, respectively). Although total and differential leukocyte counts, and phagocytic activity of neutrophils were not significantly different between the groups, superoxide productivity of neutrophils in the male athlete group was higher than that in the control group (p<0.05).Since significant differences were observed in hemoglobin and serum protein levels in the male athletes, the training is considered to have been intense. An increase was noted in the neutrophil superoxide production along with these changes, but no significant difference was observed in the phagocytic activity. Therefore, there is the possibility of superoxide overproduction, which may lead to tissue damage.
我们进行这项研究是为了评估训练对人类中性粒细胞功能的慢性影响。26 名大学生（14 名男性和 12 名女性），平均年龄 19.1±0.8 岁，根据书面问卷的回答分为运动员组和对照组。在没有训练活动的情况下，测量静息状态下循环中性粒细胞吞噬细菌（吞噬作用）和产生超氧化物（硝基四唑蓝还原）的能力。此外，我们还测量了血液学和血清生化参数。本分析显示，运动员组主观感染易感性症状（男性和女性总和）的频率高于对照组（p<0.05）。在男性调查中，运动员组的血红蛋白和血清蛋白水平低于对照组（p<0.01，p<0.05）。尽管两组之间的总白细胞和分类白细胞计数以及中性粒细胞的吞噬活性没有显著差异，但男性运动员组的中性粒细胞超氧化物产生能力高于对照组（p<0.05）。由于男性运动员的血红蛋白和血清蛋白水平存在显著差异，因此训练被认为强度很大。随着这些变化，中性粒细胞超氧化物的产生增加，但吞噬活性没有显著差异。因此，有可能发生超氧化物过度产生，这可能导致组织损伤。
