Tsukamoto K, Machida K, Ina Y, Kuriyama T, Suzuki K, Murayama R, Saiki C
Department of Hygiene and Public Health, School of Human Sciences, Waseda University, Tokorozawa, Japan.
Nihon Eiseigaku Zasshi. 1994 Oct;49(4):827-36. doi: 10.1265/jjh.49.827.
The effects of several types of crowding on immune functions were studied in mice. This study consisted of two experiments. Experiment one: Male BALB/c mice were initially housed in groups of four mice per cage. After fourteen days of acclimation, the mice were randomly divided into three groups, Control (four mice per cage, control group), Crowd-I (four mice per small space) and Crowd-II (sixteen mice per cage). These conditions were maintained for seven days. The results of experiment one were as follows: (1) The percentage of lymphocytes in the blood of Crowd-II was significantly lower than that of Control (p < 0.05). (2) The percentage of neutrophils and the absolute number of neutrophils in blood of Crowd-II were significantly higher than in Control (p < 0.05). (3) Superoxide production activity (NBT reduction activity) of blood neutrophils in Crowd-II tended to be depressed, and phagocytic activity of neutrophils was significantly depressed in Crowd-II as compared with Control (p < 0.01). These results suggest that the complexity of interrelationships among mice caused by an increase in the number of animals per cage is a very important stress factor. Experiment two: Male BALB/c mice were initially housed in groups of five mice per cage. After fourteen days of acclimation, the mice were divided into three groups, Control (five mice per cage, control group), Crowd-(1) (five mice per cmall space) and Crowd-(2) (twenty mice per cage). In Control and Crowd-(1), the same mice were used as in the acclimation period. These conditions were maintained for seven days. In this period, on the second day, all the mice were injected intraperitoneally with sheep red blood cells (SRBC). The results of experiment two were as follows: (1) The specific humoral immune response to SRBC was investigated in terms of the number of PFC in the spleens and hemagglutination in sera, but significant differences were not found among the groups. (2) Plasma IgG levels in the Crowd-(1) were significantly higher than those in Control (p < 0.05). (3) Both superoxide production activity and phagocytic activity of neutrophils were significantly depressed in Crowd-(2) as compared with Control (p < 0.01, respectively), whereas each neutrophil function of Crowd-(1) tended to be enhanced as compared with Control.
在小鼠中研究了几种拥挤类型对免疫功能的影响。本研究包括两个实验。实验一：雄性BALB/c小鼠最初每笼饲养4只。适应14天后，将小鼠随机分为三组，对照组（每笼4只小鼠，对照组）、拥挤-I组（每小空间4只小鼠）和拥挤-II组（每笼16只小鼠）。这些条件维持7天。实验一的结果如下：（1）拥挤-II组血液中淋巴细胞百分比显著低于对照组（p<0.05）。（2）拥挤-II组血液中中性粒细胞百分比和中性粒细胞绝对数显著高于对照组（p<0.05）。（3）拥挤-II组血液中性粒细胞的超氧化物产生活性（NBT还原活性）有降低趋势，且与对照组相比，拥挤-II组中性粒细胞的吞噬活性显著降低（p<0.01）。这些结果表明，每笼动物数量增加导致的小鼠间相互关系的复杂性是一个非常重要的应激因素。实验二：雄性BALB/c小鼠最初每笼饲养5只。适应14天后，将小鼠分为三组，对照组（每笼5只小鼠，对照组）、拥挤-(1)组（每平方厘米空间5只小鼠）和拥挤-(2)组（每笼20只小鼠）。对照组和拥挤-(1)组使用与适应期相同的小鼠。这些条件维持7天。在此期间，第二天，所有小鼠腹腔注射绵羊红细胞（SRBC）。实验二的结果如下：（1）根据脾脏中PFC数量和血清中的血凝反应研究对SRBC的特异性体液免疫反应，但各组间未发现显著差异。（2）拥挤-(1)组血浆IgG水平显著高于对照组（p<0.05）。（3）与对照组相比，拥挤-(2)组中性粒细胞的超氧化物产生活性和吞噬活性均显著降低（分别为p<0.01），而拥挤-(1)组的每个中性粒细胞功能与对照组相比有增强趋势。
