Botina S G, Koroban N V, Klimina K M, Glazova A A, Zakharevich N V, Zinchenko V V, Danilenko V N
Genetika. 2010 Dec;46(12):1589-97.
The species and strain genetic diversity of bacterial cultures belonging to the genus Lactobacillus, which were isolated from the gastrointestinal microbiome of the human population living in the former Soviet Union in the years 1960-1980, was studied. The bacteria demonstrated probiotic characteristics. Phylogenetic analysis of sequences of the gene coding for 16S rRNA detected earlier by us, showed that the gene found in bacteria isolated from the intestinal content of healthy adults and represented by species L. plantarum, L. helveticus, L. casei/paracasei, L. rhamnosus, and L. fermentum has high homology (97-100%) with this gene in type representatives of the species. The genotypic and strain diversity of cultures was studied using RAPD-PCR and nonspecific primers. This method with the use of the ERIC-1 primer gave reliable and reproducible results as compared that using with M13 and MSP primers and allowed the identification of examined bacteria belonging to the genus Lactobacillus at the level of species and certification at the strain level.
对1960年至1980年间从前苏联居民的胃肠道微生物群中分离出的属于乳杆菌属的细菌培养物的物种和菌株遗传多样性进行了研究。这些细菌表现出益生菌特性。我们之前对编码16S rRNA的基因序列进行的系统发育分析表明，从健康成年人肠道内容物中分离出的细菌中发现的该基因，以植物乳杆菌、瑞士乳杆菌、干酪乳杆菌/副干酪乳杆菌、鼠李糖乳杆菌和发酵乳杆菌为代表，与该物种的模式代表中的该基因具有高度同源性（97-100%）。使用RAPD-PCR和非特异性引物研究了培养物的基因型和菌株多样性。与使用M13和MSP引物相比，使用ERIC-1引物的这种方法给出了可靠且可重复的结果，并允许在物种水平上鉴定所检测的属于乳杆菌属的细菌，并在菌株水平上进行认证。
