Liu Man, Hu Xin, Wang Xu, Zhang Jingjing, Peng Xubing, Hu Zhigang, Liu Yifei
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
Hubei Kangnong Seed Co., Ltd., Yichang, China.
Front Plant Sci. 2020 Dec 23;11:600249. doi: 10.3389/fpls.2020.600249. eCollection 2020.
is an important medicinal plant in Chinese traditional medicine. Its roots, which are known as in Chinese, are broadly applied to treat inflammation, arthritis, and headache. With increasing market demand, the wild resources of have been overexploited, and conservation, assessment of genetic resources and breeding for this species is needed. Here, we sequenced the transcriptome of and developed simple sequence repeat (SSR) markers from it to construct a core collection based on 208 samples collected from Changyang-related regions. A total of 132 alleles were obtained for 17 SSR loci used with the polymorphic information content () ranging from 0.44 to 0.83. Abundant genetic diversity was inferred by Shannon's information index (1.51), observed (0.57) and expected heterozygosity (0.72). The clustering analysis resulted into two sample groups and analysis of molecular variance (AMOVA) showed only 6% genetic variation existed among populations. A further metabolic analysis of these samples revealed the main coumarin contents, such as osthole and columbianadin. According to the genetic and metabolic data, we adopted the least distance stepwise sampling strategy to construct seven preliminary core collections, of which the 20CC collection, which possessed 42 individuals accounting for 90.20% of the genetic diversity of the original germplasm, represented the best core collection. This study will contribute to the conservation and management of wild germplasm resources and provide a material basis for future selection and breeding of this medicinal plant.
是中国传统医学中的一种重要药用植物。其根在中国被称为，广泛用于治疗炎症、关节炎和头痛。随着市场需求的增加，该植物的野生资源已被过度开发，因此需要对其进行保护、遗传资源评估和育种。在此，我们对该植物的转录组进行了测序，并从中开发了简单序列重复（SSR）标记，以基于从长阳相关地区收集的208个样本构建核心种质库。使用的17个SSR位点共获得132个等位基因，多态信息含量（）范围为0.44至0.83。通过香农信息指数（1.51）、观察杂合度（0.57）和期望杂合度（0.72）推断出丰富的遗传多样性。聚类分析产生了两个样本组，分子方差分析（AMOVA）表明群体间仅存在6%的遗传变异。对这些样本的进一步代谢分析揭示了主要香豆素成分，如蛇床子素和哥伦比亚内酯。根据遗传和代谢数据，我们采用最小距离逐步抽样策略构建了7个初步核心种质库，其中20CC种质库包含42个个体，占原始种质遗传多样性的90.20%，代表了最佳核心种质库。本研究将有助于该植物野生种质资源的保护和管理，并为该药用植物未来的选育提供物质基础。
