Bomsel M, Parton R, Kuznetsov S A, Schroer T A, Gruenberg J
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Cell. 1990 Aug 24;62(4):719-31. doi: 10.1016/0092-8674(90)90117-w.
The pathways of endocytosis from the apical and the basolateral domains of epithelial MDCK cells are known to converge at the level of late endosomes in vivo. We have now reconstituted the meeting process in a cell-free assay that measures the fusion of apically and basolaterally derived endocytic vesicles with late endosomes. Our results show that this in vitro process requires the presence of polymerized microtubules, as does the convergence of the two pathways in vivo, and also depends on the presence of microtubule binding proteins, in particular the mechanochemical motors kinesin and cytoplasmic dynein.
已知上皮性MDCK细胞顶端和基底外侧区域的内吞作用途径在体内晚期内体水平会合。我们现在已经在无细胞测定中重建了会合过程，该测定测量顶端和基底外侧来源的内吞小泡与晚期内体的融合。我们的结果表明，这种体外过程需要聚合微管的存在，体内两条途径的会合也是如此，并且还依赖于微管结合蛋白的存在，特别是机械化学马达驱动蛋白和胞质动力蛋白。
